微卫星多态性检测技术及其在保护遗传学中的应用  

Detection technique of microsatellites polymorphism and its application in conservation genetics.

作　　者：晏鹏[1] 吴孝兵[1] 史燕[1] 张方[1] 

机构地区：[1]安徽师范大学生命科学学院,芜湖241000

出　　处：《应用生态学报》2003年第3期461-464,共4页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目 (3 0 2 70 2 13 );安徽省自然科学基金项目(0 10 43 5 0 1);安徽省学术与技术带头人基金资助项目

摘　　要：微卫星是一种新型的分子遗传标记 ,具有只需极少量样品即可进行PCR扩增等优点 ,使之极其适合于保护遗传学研究 .阐释了微卫星的结构、微卫星多态检测技术的原理 ,简要论述了微卫星多态检测技术的优点及其应用 。Microsatellites are a novel molecular genetic marker,and offer unusual advantages,e.g., with the using of a very few sample, they can be amplified by PCR,which are particularly appropriate in the research of conservation genetics. In this paper,the structure of microsatellites and the principle of the detection technique of microsatellites polymorphism were illustrated, and the advantages and the application of this detection technique were described briefly. For the isolation of microsatellite loci in animals,an enrichment method was introduced. And then,some practical problems of this technique were summarized.

关 键 词：微卫星DNA 多态性 保护遗传 

分 类 号：Q953[生物学—动物学]