测定血清苯巴比妥浓度2种差示分光光度法比较  

Determination of phenobarbital in serum by two methods of differential spectrophotometry and their comparison

作　　者：王刚[1] 谷容[1] 张振琪[1] 刘彬[1] 

机构地区：[1]重庆医科大学儿童医院,重庆400014

出　　处：《中国医院药学杂志》2003年第3期137-139,共3页Chinese Journal of Hospital Pharmacy

摘　　要：目的 :建立 2种条件下的差示分光光度法测定血清苯巴比妥浓度的方法并进行比较。方法 :在酸性条件下以三氯甲烷从血清中二次提取 ,利用苯巴比妥在强碱与弱碱及弱碱与酸性介质中吸收度差值与浓度有良好的线性 ,分别按不同条件下的差示分光光度法测定血清中苯巴比妥浓度。结果 :两种方法血清苯巴比妥浓度的线性范围 2～ 80mg·L- 1 ,在弱碱与酸性的条件下 ,其回收率和RSD分别为 (1 0 0 .7± 0 .4) % ,(2 .1 7± 0 .0 8) % ,血清苯巴比妥在此条件下 2 5min内较稳定 ,对测定结果无影响。在强碱与弱碱性的条件下 ,回收率和RSD分别是 (99.9± 0 .9) %和 (4.73± 0 .1 8) % ,血清中提取的苯巴比妥在 1 0min时存在显著影响 ,1 5min吸收度较最初降低了 9%。结论 :按弱碱与酸性条件下的差示分光光度法测定血清苯巴比妥 。OBJECTIVE To develop and compare two methods for determining the contents of phenobarbital in serum by differential spectrophotomentry under two conditions.METHODS Phenobarbital in serum was extracted two times with trichloromethane under acidic condition. The standard curves of phenobarbital in serum were established according to the absorbance deviations of phenobarbital in strong base and weak base, weak base and acidic media ,and the phenobarbital in serum was determined by differential spectraphotometry under differential conditions.RESULTS The linear range of phenobarbital in serum under two conditions were 2～80 mg·L -1 .The average recovery and RSD were ( 100.7 ± 0.4 )%, ( 2.17 ± 0.08 )% under weak base and acidic condition, and the result of determination of phenobarbital in serum was stable in 25 min. The average recovery and RSD were ( 99.9 ± 0.9 )%, ( 4.73 ± 0.18 )% under strong base and weak base condition, and the result of determination of phenobarbital in serum was seriously influenced in 10 min. The absorbance of phenobarbital is 9% lower than at that time.CONCLUSIONS The determination of phenobarbital in serum by differential spectrophotomatry under weak base and acidic conditions is stable and accurate.

关 键 词：苯巴比妥 差示分光光度法 血清浓度 

分 类 号：R927[医药卫生—药学]