人CD4^+CD25^+T细胞和CD4^+CD25^-T细胞蛋白质组的双向电泳分析  

Proteome of human CD4^+CD25^+T cells vs CD4^+CD25^-T cells: Comparative analysis by two dimensional electrophoresis

作　　者：彭双发[1] 吴军[1] 殷剑宁[2] 郑峻松[1] 王贵波[1] 贺伟峰[1] 陈希炜[1] 易绍萱[1] 袁军[1] 解志杰[1] 

机构地区：[1]第三军医大学附属西南医院全军烧伤研究所,重庆400038 [2]北京华大基因研究中心,北京101300

出　　处：《第三军医大学学报》2003年第6期492-494,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金重大资助项目 ( 399934 30 2 ) ;国家自然科学基金面上资助项目 ( 30 2 0 0 2 6 4)

摘　　要：目的　用双向电泳技术分析人CD4+ CD2 5 + T细胞和CD4+ CD2 5 -T细胞表达的蛋白质组 ,建立二者之间的差异表达谱。方法　从人脾脏分选CD4+ CD2 5 + T细胞和CD4+ CD2 5 -T细胞 ,用双向电泳技术分析两种细胞蛋白质组的表达差异。结果　分选细胞纯度分别为CD4+ CD2 5 + T细胞 82 .3 % ,CD4+ CD2 5 -T细胞 96.5 % ,双向电泳分析发现有 4个蛋白点明显仅于CD4+ CD2 5 + T细胞检测到表达 ,有 9个蛋白点明显仅于CD4+ CD2 5 -T细胞检测到表达。结论　CD4+ CD2 5 + T细胞和CD4+ CD2 5Objective To comparatively analyze the proteomes of human CD4 +CD25 +T cells and CD4 +CD25 -T cells. Methods CD4 +CD25 +T cells and CD4 +CD25 -T cells were isolated from human spleen and their purity was identified. The difference of protein expression in CD4 +CD25 +T cells and CD4 +CD25 -T cells was analyzed by two dimensional electrophoresis. Results The purity of CD4 +CD25 +T cells and CD4 +CD25 -T cells was 82.3% and 96.5%, respectively. By means of two dimensional electrophoresis, there were 4 protein spots exclusively detected in CD4 +CD25 +T cells while 9 protein spots in CD4 +CD25 -T cells. Conclusion There is significant difference between proteomes of human CD4 +CD25 +T cells and CD4 +CD25 -T cells.

关 键 词：CD4^+CD25^+调节性T细胞 蛋白质组 双向电泳 

分 类 号：R392－33[医药卫生—免疫学] R392.11[医药卫生—基础医学]