缺氧条件下梭曼中毒对大鼠心肌M_2受体mRNA表达的影响  

Effect of soman intoxication under hypoxia on the expression of M_2R mRNA of rat myocardium

作　　者：刘辉[1] 刘勇[1] 王仕丽[1] 赵吉清[1] 吴强[1] 

机构地区：[1]第三军医大学预防医学系毒理学教研室,重庆400038

出　　处：《第三军医大学学报》2003年第6期508-510,共3页Journal of Third Military Medical University

摘　　要：目的　探讨缺氧条件下梭曼中毒大鼠心肌M2 胆碱能受体表达的变化。方法　采用半定量RT PCR法分别检测缺氧、梭曼中毒以及缺氧条件下梭曼中毒大鼠心肌M2 受体mRNA的表达。结果　与正常对照组比较 ,缺氧组大鼠心肌M2 受体mRNA表达增强 ,但相差并不显著 ;单纯梭曼中毒组和缺氧中毒组大鼠M2 受体在致伤后各时间点表达明显减弱 ,但与单纯梭曼中毒组比较 ,缺氧中毒组大鼠心肌M2 受体mRNA的表达在致伤后 12h和 2 4h明显增强 (P <0 .0 5 )。结论　M2 受体对有机磷毒剂非常敏感 ,缺氧条件下大鼠心肌M2 受体的表达相对于平原中毒组有所增强 。reression of M 2 receptor mRNA of rat myocardium intoxicated by soman under high altitude hypoxia. Methods The expressions of M 2 receptor mRNA under hypoxia, soman intoxication and soman intoxication under hypoxia were detected by semi quantitative RT PCR, respectively. Results The expression of M 2 receptor mRNA increased in the high altitude hypoxia group. Both simple soman intoxication and combined soman intoxication and hypoxia decreased the expression of M 2 receptor rapidly. But under hypoxia, the expression increased significantly at 12 h and 24 h. Conclusion M 2 receptor was sensitive to nerve agents. Compared to simple soman intoxication group, the expression of M 2 receptor increased in combined soman intoxication and hypoxia group. This may be one of the major factors leading to aggravation of the injury of heart function by nerve agents in high altitude area.

关 键 词：缺氧 梭曼中毒 RT-PCR M2受体 

分 类 号：R392.13[医药卫生—免疫学] R595.9[医药卫生—基础医学]