机采血小板P-选择素在常规和冰冻保存条件下的表达研究  

Expression of P-selectin on routinely stored or cryopreserved platelets obtained by single donator apheresis

作　　者：赵树铭[1] 成晓玲[1] 李书武[1] 刁荣华[1] 刘璐[1] 张红[1] 陈寒梅[1] 陈敏[1] 

机构地区：[1]第三军医大学附属西南医院输血科,重庆400038

出　　处：《第三军医大学学报》2003年第6期525-527,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目 ( 30 0 0 0 0 85)

摘　　要：目的　探讨常规和冰冻保存条件下对血小板功能的影响。方法　 10单位机采血小板分别用常规保存袋和低温保存袋贮存 ,前者贮存 5d ,每天留取标本 ,后者于 -85℃贮存后 5d复苏留样。分别检测血小板聚集率、血小板计数、pH值和血小板膜糖蛋白P 选择素的变化。结果　常规保存条件下 ,5d内血小板聚集率、pH、血小板计数均无显著性变化 ,血小板P 选择素在 1d后即明显升高 (从 3 .77± 1.86到 10 .87± 4.75 ,达 3倍 ,P <0 .0 1)。冰冻保存时 ,血小板聚集率、pH、血小板计数无显著性变化 ,P 选择素的表达在冰冻后呈显著升高 (从 4.3 7± 1.82到 16.88± 7.2 3 ,达 4倍 ,P <0 .0 1)。结论　血小板P 选择素在常规和冰冻保存条件下的表达变化提示血小板离体后被活化 。Objective To explore the influence of routine storage or cryopreservation on platelet function. Methods Ten units of platelets were collected by single donator apheresis. Each one was divided into 2 equal parts which were preserved with routine storage and cryopreservation respectively. The samples of platelet were respectively collected on each day during the 5 days' routine storage and before and after 5 days of cryopreservation. The platelet aggregative rate, platelet count and pH value were detected with routine methods. The expression of P selectin on the platelet samples was measured by flow cytometry. Results In routine storage group, the platelet aggregative rate, platelet count and pH value had no change in the 5 days, and the expression of P selectin increased significantly from 3.77±1.86 to 10.87±4.75 ( P <0.01) on the first day of storage and remained higher levels thereafter. In the cryopreservation group, no change was found in the parameters mentioned above, and the expression of P selectin was found to increase significantly from 4.37±1.82 to 16.88±7.23 ( P <0.01) after cryopreservation. Conclusion The expression of P selectin on platelets is found to increase after collection under either the routine storage or cryopreservation, and thus it can be regarded as a sensitive marker for platelet activation.

关 键 词：P-选择素 机采血小板 常规保存 冰冻保存 

分 类 号：R318.52[医药卫生—生物医学工程] R331.143[医药卫生—基础医学]