维生素C、E对中华绒螯蟹生殖性能的影响  

Effect of ascorbic acid and α-tocopherol in broodstock diet on reproductive performance of Eriocheir sinensis

作　　者：艾春香[1] 陈立侨[1] 周忠良[1] Glenn Y Deng 江洪波[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062 [2]NuGen Technologies

出　　处：《水产学报》2003年第1期62-68,共7页Journal of Fisheries of China

基　　金：国家自然科学基金项目(30271012);上海市"曙光计划"(975G06)基金项目;高等学校博士点专项基金项目(2000026903);教育部回国留学人员科研启动基金项目;霍英东青年教师基金项目部分资助

摘　　要：通过对河蟹雌性亲体投喂不添加或添加VC、VE的4组实验饲料,并以新鲜河蚌为对照,进行198d饲养试验,以雌蟹性腺系数、产卵力、孵化率以及各组织中SOD活性、MDA含量等指标作为判据,研究了VC、VE对雌蟹生殖性能的影响。结果表明,1(投喂添加0.5mg·(100g)-1VC饲料组)、2(投喂添加0.022mg·(100g)-1VE饲料组)和3(投喂添加0.5mg·(100g)-1VC饲料和0.022mg·(100g)-1VE饲料组)3个组雌蟹的产卵力分别为3620个卵细胞·g-1体重、3540个卵细胞·g-1体重、3710个卵细胞·g-1体重,孵化率分别是83.03%、84.06%、86.27%,均极显著高于4组(投喂未添加维生素饲料组)雌蟹的产卵力(2490个卵细胞·g-1体重)和孵化率(29.28%)(P<0.01),也显著高于5组(饲喂河蚌)雌蟹的产卵力(3010个卵细胞·g-1体重)和孵化率(71.12%)(P<0.05)。雌蟹卵巢中SOD活性,1、2和3组则比4组和5组显著低(P<0.05),分别为56.35Nu·mL-1、53.61Nu·mL-1和39.87Nu·mL-1,相应地,MDA含量分别为6.03nmol·mL-1、5.65nmol·mL-1和5.57nmol·mL-1;4组雌蟹,虽然其卵巢中SOD活性较高,达79.25Nu·mL-1,但因饲料中缺乏抗氧化性的VC、VE,引发卵子脂质过氧化,MDA含量显著增加(P<0.05),为23.18nmol·mL-1,导致卵质低下,从而影响了卵子孵化。综上所述。Cultured trials lasting 198 days were conducted to determine the physiological effects of dietary vitamin C and E on reproductive performance of the Chinese mittenhanded crabs Eriocheir sinensis, with mean weight of 78.21±25.68g, and female broodstocks were fed with four experimental diets of varying composition. The control group was fed with fresh clams. The gonadosomatic index (GSI), fecundity, hatchability of eggs, activities of SOD and the content of MDA in the different tissues, were used to evaluate the performance of the crabs fed with different diets. The results showed that the fecundity and hatchability of groups 1, 2 and 3 were 3 620 cell·g-1(body weight) and 83.03%; 3 540 cell·g-1 (body weight) and 84.06% and 3 790 cell·g-1(body weight) and 86.27% respectively, which were very significantly higher than those of group 4 (2 490 cell·g-1 body weight and 29.28%) (P<0.01), and those of group 5 (3 010 cell·g-1 body weight and 71.12%)(P<0.05). The SOD activities in ovaries of groups 1, 2 and 3 were 56.35Nu·mL-1, 53.61Nu·mL-1 and 39.87Nu·mL-1 respectively, significantly lower than those of group 4 and 5 (P<0.05), and MDA contents were 6.03nmol·mL-1, 5.65nmol·mL-1 and 5.57nmol·mL-1 respectively. Though the SOD activity in ovaries of group 4 was 79.25Nu·mL-1, peroxidation still took place because of deficiency in VC and VE, so MDA content in ovaries of group 4 was high, 23.18nmol·mL-1, resulting in very low hatchability of group 4. This experiment demonstrated that VC, VE supplemented in the diets of female broodstocks can improve the reproductive performance.

关 键 词：中华绒螯蟹 维生素C、E 生殖营养需求 生殖性能 

分 类 号：S963[农业科学—水产养殖]