耐盐红螺菌对水产养殖病害细菌的拮抗作用  

The antagonistic action of salt-resistant Rhodospirillaceae bacteria on aquacultural pathogens

作　　者：邱宏端[1] 陈智伟[1] 袁重桂[1] 林娟[1] 高敏珠 

机构地区：[1]福州大学生物与食品科学工程系,福建福州350002 [2]福州市科技馆,福建福州350004

出　　处：《水产学报》2003年第1期69-74,共6页Journal of Fisheries of China

基　　金：福建省自然科学基金项目(B0210004);福建省教育厅项目(JB00074);福州大学科技发展基金项目(XKJ(QD)0133)

摘　　要：为探明光合细菌对水产养殖动物病害的生物防治功能,对病鳖、鳗及南美白对虾动物体上的病原菌进行分离、纯化与鉴定,人工感染健康动物检测致病性;探讨耐盐红螺菌对病害细菌分离株的抑菌效能,和有效抑菌作用时细胞所处的生长期。试验结果表明,水产动物病害细菌分离株为弧菌属,气单胞菌属和假单胞菌属菌株;耐盐红螺菌的代谢产物对病害细菌分离株均有抑制作用,其平板抑菌圈为1.2～1.4cm,最低抑菌浓度多为4～8倍稀释液;耐盐红螺菌的拮抗物质随细胞对数生长过程中生成,至细胞衰亡期达到最大值。The pathogens from ill turtles, eels and Ictiobus cytrinellus were separated, purified and identified to prove up the biocontrol function of Photosynthetic bacteria(PSB) on diseases of aquatic animals. The pathogenicity of pathogens is examined by artificially infecting the healthy animals. And the antibacterial abilities of saltresistant Rhodospirillaceae bacteria against those separated pathogens were studied. At the same time, the phases that PSB produces effective antibacterial action were determined. The results show that the separated pathogens from aquatic animals are Vibrio, Aeromonas, and Pseudomonas. The metabolic products of saltresistant Rhodospirillaceae bacteria show the bacteriostastic activity to those separated pathogens: the diameter of antibacterial zone is 1.2-1.4cm, the minimal inhibitory concentrations (MIC) are mostly at the diluted times 4-8. The antagonistic substances of saltresistant Rhodospirillaceae bacteria are produced in the exponential phase initially and reached maximal concentration in the contabescence phase.

关 键 词：耐盐红螺菌 水产养殖 病害细菌 拮抗作用 

分 类 号：S94[农业科学—水产养殖]