禽副粘病毒2型(PMV-2)群特异性单克隆抗体的研究  

The Preparation and Identification of Monoclonal Antibodies Against Avian Paramyxovirus Type2

作　　者：张国中[1] 赖志[1] 赵继勋[1] 

机构地区：[1]中国农业大学实验动物研究所,北京100094

出　　处：《畜牧兽医学报》2003年第2期188-190,共3页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：禽副粘病毒 2型 (Yucaipa株 )鸡胚成纤维细胞适应毒经差速离心和蔗糖密度梯度离心法提纯 ,免疫Balb/c小鼠 ,取脾细胞与SP2 / 0骨髓瘤细胞融合 ,经间接ELISA方法筛选 ,有限稀释 3次克隆 ,获得了两株分泌PMV 2单克隆抗体的杂交瘤细胞株 6E9和 7E5。经鉴定两株单抗均为IgG1亚类 ,杂交瘤细胞的平均染色体分别为 96条和98条。细胞培养上清及腹水效价分别为 1∶2 5 6 0 0、1∶5 12 0 0和 1∶10 5、1∶10 7。 6E9和 7E5单抗能稳定分泌抗体且与NDV、AIV、EDSV无交叉反应。间接ELISA实验表明 4株PMV 2 (Yucaipa株、F4 株、F6株和F8株 )均含有单抗6E9和 7E5所针对的位点。By fusing the SP2/0 myeloma cells with the spleen cells of purified avian paramyxovirus type 2(PMV-2) immunized mouse,two hybridoma cell lines which secret monoclonal antibodies (McAb) against PMV-2 were established after screening by ELISA and cloning three times by limiting dilution.The immunoglobulin subclass of two McAbs were IgG1.The ELISA titers of culture superatant and ascities were 1∶ 25 600?1∶ 51 200 and 1∶105?1∶107 respectively.They did not cross react with the NDV?AIV?EDSV,showed good specificity.The results of indirect ELISA showed that all four viruses contained the recognized epitopes of two McAbs.

关 键 词：禽副粘病毒2型 单克隆抗体 群特异性 

分 类 号：S852.65[农业科学—基础兽医学]