尿路移行上皮细胞的培养鉴定  

Culture and identification of urothelial transitional epithelium

作　　者：耿红全[1] 陈方[1] 吴湘如 周昕[1] 谢华[1] 陆建华[1] 

机构地区：[1]上海第二医科大学附属新华医院上海儿童医学中心儿外科,上海200092 [2]新华医院病理科

出　　处：《临床儿科杂志》2003年第2期114-115,共2页Journal of Clinical Pediatrics

基　　金：国家自然科学基金 (301709361);上海市教委重点科研基金 (99ZD06);上海市教委曙光计划资助

摘　　要：为建立尿路上皮细胞分离、培养、扩增的常规 ,获得构建组织工程学泌尿系器官所需的大量尿路移行上皮细胞 ,分别采用刮削法和组织块法分离出尿路上皮细胞 ,在角化细胞培养基 (Keratinocyte_SFM)中培养 ,观察细胞形态、扩增情况 ,并用免疫组化方法检测细胞类型特异性蛋白质。结果 :尿路移行上皮细胞生长良好 ,呈现出典型的移行上皮形态特征。细胞经10次传代后仍扩增迅速 ,免疫组化染色显示CKAE1/AE3 阳性、CK34βE12 阴性。提示该常规中细胞分离方法可靠 ,传代顺利 ,扩增迅速 ;尿路上皮细胞经10次传代仍保持其固有生物学特性 。To establish a protocol for isolating,culturing,proliferating urothelial cells,and to obtain large number of autologous urothelial cells for tissue_engineered substitutes.Urothelial cells were isolated from sample tissues by scraping or tissue explanting and were cultured in Karetinocyte_SFM.Morphology and expansion of the cells were observed.Cell_type specific proteins were analyzed by immunohistochemical methods.The results showed that urothelial cells presented a typical morphological feature of transitional epithelium.Cells could be passaged 10 times without a noticeable decrease in rate of cell proliferation.Immunohistochemistry showed that CKAE1/AE3 was positive and CK34βE12 was negative.Therefore,in this protocol,the isolation of urothelial cells are simple,the cells proliferate rapidly and can be passaged easily.The biological properties of urothelial cells have been kept and no signs of senescence were seen even after the 10th passage.

关 键 词：尿路 移行上皮细胞 培养鉴定 细胞培养 

分 类 号：R318.0[医药卫生—生物医学工程]