日本血吸虫重组抗原rSjGST-32诱导小鼠保护性免疫效果的比较  

Protective Effect Against Schistosoma japonicum of Recombinant Fusion Protein SjGST-32 in Mice

作　　者：田明礼[1] 蔡春[1] 易新元[1] 曾宪芳[1] 张顺科[1] 

机构地区：[1]中南大学湘雅医学院寄生虫学教研室

出　　处：《中国寄生虫学与寄生虫病杂志》2003年第1期24-26,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的　探索日本血吸虫重组抗原 r Sj GST- 32适合的免疫剂量。　方法　以 5 0、10 0、2 0 0μg 3个不同剂量r Sj GST- 32加佐剂皂角甙 A (Quil A) 5 0μg免疫 BAL B/ c小鼠 ,同时设 Quil A和 PBS对照组。EL ISA法检测抗体水平。末次免疫 4 wk后攻击感染 ,用减虫率及减卵率表示保护力。　结果　与 PBS对照组比较 ,5 0、10 0、2 0 0μg r Sj GST- 32组的减虫率分别为 38.1% ,4 7.8%和 4 8.8% ;每克肝卵 (L EPG)减少率分别为 39.1% ,5 3.5 %和 5 3.6 % ;每条雌虫每克肝所含虫卵数 (L EPF)减少率分别为 2 2 .5 % ,2 2 .8%和 2 1.8% ;抗体滴度分别达 1∶ 5 12 0 0 ,1∶ 10 2 4 0 0和1∶ 10 2 4 0 0。　结论　重组抗原 r Sj GST- 32加 Quil A佐剂免疫小鼠 ,以 10 0 μg r Sj GST- 32剂量较为适合。Objective To explore the optimal immune doses of recombinant antigen rSjGST\|32, with QuilA as \{adjuvant\}. \ Methods\ BALB/c mice were immunized with doses of 50, 100 and 200 μg rSjGST\|32/mouse plus 50 μg QuilA adjuvant. QuilA and PBS were used as controls. Levels of specific antibodies were detected by ELISA. The mice were \{challenged\} 4 weeks after last vaccination. Worms and eggs collected from the livers of mice were counted 45 days \{after\} \{challenge\}. \ Results \ As compared with the control groups, the worm reduction rate in the 50, 100 and 200 μg \{experiment\} groups was 38\^1%, 47\^8% and 48\^8%, respectively. The reduction rate of liver eggs per gram (LEPG) was 39\^1%, 53\^5% and 53\^6%, respectively, and the reduction rate of the liver eggs per female (LEPF) was 22\^5%, 22\^8% and 21\^8%, respectively. The specific antibody titers in sera reached 1∶\{51 200\}, 1∶\{102 400\} and 1∶\{102 400\}, \{respectively\} before challenge. \ Conclusion \ The results show that for vaccinating BALB/c mice, the optimal antigen dose is 100 μg of recombinant rSjGST\|32 plus QuilA adjuvant.

关 键 词：日本血吸虫 重组抗原 rSjGST-32 小鼠 免疫效果 抗原剂量 

分 类 号：R532.21[医药卫生—内科学] R392.11[医药卫生—临床医学]