反相高效液相色谱法测定黄芪中黄芪甲苷的含量  

Determination of astragaloside Ⅳ in the Radix Astragali by RP-HPLC

作　　者：李欢欣[1] 郝桂明[2] 赵春杰[2] 郝桂彤 

机构地区：[1]锦州市中心医院,辽宁锦州121001 [2]沈阳药科大学药物分析教研室,辽宁沈阳110016 [3]天津怀仁制药有限公司,天津300193

出　　处：《中国药学杂志》2003年第3期212-213,共2页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目 ( 39970 910 )

摘　　要：目的　建立黄芪中主要有效成分黄芪甲苷的RP HPLC。方法　采用KromasilC18( 2 5 0mm× 4.6mm ,5 μm)色谱柱 ,乙腈 水 ( 3 0∶70 )为流动相 ,检测波长为 2 0 3nm ,以卡马西平为内标。结果　线性范围为 0 .1～ 1.0mg·mL-1,三水平的平均回收率 ( % )分别为 82 .1,85 .4和 88.4,RSD( % )分别为 2 .88,1.3 1和 2 .67。结论　本法准确 ,可靠 。OBJECTIVE To establish a reversed-phase high-performance liquid chromatographic method for the determination of astragaloside Ⅳ,which was known as the active constituent of Radix Astragali.METHOD Acetonitrile-water(30∶70) was used as the mobile phase on a Kromasil C 18 column.The detector was operated at 203 nm and carbamazepine was added as the internal standard.RESULTS The method was carried out over the range of 0.1～1.0 mg·mL -1 for astragaloside Ⅳ.The recoveries(%) were 82.1,85.4 and 88.4 with the RSD 2.88,1.31 and 2.67 for three concentrations,respectively.CONCLUSION The method had good accuracy and repeatability and it can be use for the quality control of Radix Astragali.

关 键 词：反相高效液相色谱 黄芪 黄芪甲苷 

分 类 号：R282.71[医药卫生—中药学]