两种人工骨的构建及其在体内诱导成骨能力的比较  

The Construction of Two Kinds of Artificial Bone and the Comparison of Their Osteogenesis Capability in vivo

作　　者：张其清[1] 张立海[1] 刘玲蓉[1] 梁屹[1] 任百智[1] 王福君[1] 

机构地区：[1]中国医学科学院中国协和医科大学生物医学工程研究所生物材料研究室,天津300192

出　　处：《中国医学科学院学报》2003年第1期17-21,T003-T004,共7页Acta Academiae Medicinae Sinicae

基　　金：天津市自然科学重点基金(003802511);国家重点基础研究发展规划项目(G199054309);"十五"国家科技攻关计划(2001BA706B-177)资助~~

摘　　要：目的分别以胶原-羟基磷灰石(hydroxyapatite,HA)和胶原-HA-硫酸软骨素(chondroitinsulfate,CS)为支架构建两类人工骨,并比较其生物学性能。方法将人重组骨形态发生蛋白-2(recombinanthumanbonemorphogeneticprotein-2,rhBMP-2)吸附于两种人工骨支架体系形成具有生物活性的人工骨,将其埋入大鼠的肌肉内,每周取材进行HE染色观察,比较两种人工骨在体内诱导成骨能力的差异。结果两种人工骨均能在体内诱导新骨的形成,胶原-HA-CS人工骨在体内诱导成骨的程度优于胶原-HA人工骨。结论硫酸软骨素可以促进新骨的形成,加速骨愈合的过程。Objective To construct artificial bone with collagen-hydroxyapatite (HA)or collagen-HA-chondroitin sulfate (CS)as the scaffolds,and observe their biological properties.Methods The artificial bones were constructed by attaching recombinant human bone morphogenetic protein2(rhBMP -2)on those scaffolds.And then they were embedded into muscles of rats.Every weekend those newly formed bones were taken from muscles for comparing the difference in osteogenetic capability of two kinds of artificial bone in vivo.Results Both kinds of artificial bones could induce bone regeneration in muscle.The collagen-HA-CS artificial bone was superior to the collagen-HA artificial in bone-guided degree.Conclusions The CS could promote the form action of new bone and accelerate the bone healing.

关 键 词：胶原 羟基磷灰石 硫酸软骨素 骨形态发生蛋白 

分 类 号：R816.8[医药卫生—放射医学]