HPLC法测定钙-D颗粒剂中维生素D_3的含量  

Determination of Vitamin D_3 in Calcium-D granules by HPLC

作　　者：历士旺[1] 赵亚平[1] 张亚锋 赵润年 

机构地区：[1]天津市儿童医院,天津300074 [2]陕西省渭南市中心医院,陕西渭南714000

出　　处：《西北药学杂志》2003年第1期3-4,共2页Northwest Pharmaceutical Journal

摘　　要：目的　建立钙 - D颗粒剂中维生素 D3的含量测定方法。方法　采用 HPLC法 ,色谱柱为 C18柱 ,以正己烷-乙醇 (98∶ 2 )为流动相 ,在 2 5 4 nm波长处检测。结果　维生素 D3在 1 2～ 48IU·mg-1范围内 ,浓度与峰面积呈良好的线性关系 (r=0 .9997) ,平均回收率为 99.92 % (n =6 ,RSD =0 .89% )。结论　本方法简便可靠 ,重现性好 。Objective To establish a HPLC method for determination of Vitamin D 3 in Calcium-D granules. Method A C 18 column was used. The mobile phase consisted of hexane and ethanol(98∶2) and the detection wavelength was 254 nm. Results The linear range was between 12～48 IU·mg -1(r=0.999 7), the average recovery was 99.92%(RSD=0.89%, n=6). Conclusion This method is simple, convenient and is suitable for the quality control of Calcium-D granules.

关 键 词：钙-D颗粒剂 维生素D3 HPLC 

分 类 号：R927.2[医药卫生—药学]