重组人乳头瘤病毒6型病毒样颗粒诱导中和抗体  

Recombinant Human Papillomavirus Type 6 Virus-like Particles Induced Neutralizing Antibodies

作　　者：王淼[1] 陈连凤[2] 许雪梅[1] 王立良[1] 陈建国[1] 刘士德[1] 司静懿[1] 宋国兴[1] 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所,北京100005 [2]协和医院,北京100005

出　　处：《病毒学报》2003年第1期11-15,共5页Chinese Journal of Virology

基　　金：国家自然科学基金资助项目 ( 39780 0 11);国家高技术"86 3"计划资助项目 ( 2 0 0 1AA2 15 2 2 1)

摘　　要：为研究重组病毒样颗粒 (virus -likeparticle ,VLP)免疫血清的抗感染作用 ,用重组杆状病毒在昆虫细胞中表达制备的人乳头瘤病毒 6型 (humanpapillomavirustype 6 ,HPV - 6 )L1VLP和HPV - 6L1+L2VLP免疫BALB/c小鼠 ,获得抗血清 ,ELISA法测定抗体滴度 ,在细胞水平和裸鼠异源组织移植模型中评价了免疫血清的中和病毒抗感染作用。VLP诱导了高滴度 ( >1∶10 0 0 0 )的血清抗体 ,抗血清可以特异地阻断人胚上皮细胞对VLP的摄入 ,并且能抑制从尖锐湿疣活检标本提取的HPV对人上皮组织的感染。重组HPV - 6VLP免疫小鼠诱导的血清抗体具有中和病毒、抑制感染的作用。提示重组VLP可以用于研制HVP预防性疫苗。Human papillomaviruses(HPVs)are non-enveloped viruses with the capsid composed of major capsid protein L1 and minor capsid protein L2.To investigate the anti-infection effect of immunizing sera raised with recombinant HPV type 6(HPV-6)virus-like particles(VLP),HPV-6 L1 VLP and HPV-6 L1+L2 VLP prepared from h recombinant baculoviruses infected Sf9 cells were used to immunize BALB/c mice.The capability of the raised anti-sera to neutralize virus and inhibit infection was evaluated in cell level and in nude mouse xenograft transplant model after titer determination with ELISA.The titers of serum antibodies in mice vaccinated with both kinds of VLP were above 1∶10,000.The anti-sera specifically inhibited uptake of VLP into human embryonic epithelial cells,and neutralized infection of human foreskins by HPV isolated from genital wart biopsies.In conclusion,the prepared recombinant HPV-6 VLP induced high-titer serum antibodies that could neutralize HPV infection.Recombinant VLP can be used to explore preventive vaccine against genital warts.

关 键 词：人乳头瘤病毒6型 病毒样颗粒 中和抗体 

分 类 号：R373.9[医药卫生—病原生物学]