神经生长因子分化后的PC12细胞对乙酰胆碱的敏感性及其特性  

THE PROPERTIES AND SENSITIVITY TO ACETYLCHOLINE OF PC12 CELLS DIFFERENTIATED WITH NGF

作　　者：石丽君[1] 王可[1] 刘玲爱[1] 王春安[2] 

机构地区：[1]北京军医学院生理教研室,北京100071 [2]第二军医大学生理教研室,上海200433

出　　处：《中国应用生理学杂志》2003年第1期74-77,共4页Chinese Journal of Applied Physiology

摘　　要：目的和方法 :采用全细胞膜片钳技术观察神经生长因子 (NGF)分化后的PC1 2细胞对乙酰胆碱 (ACh)的敏感性 ,并对ACh诱发电流 (IACh)的特性进行分析。结果 :NGF处理后的PC1 2细胞不仅形态上向交感神经元分化 ,而且具有电学兴奋性 ,它对ACh敏感性比未分化前显著提高。药理学鉴定表明PC1 2上的IACh是由烟碱受体(nAChR)引起的 ,具有明显的失敏特性。宏观IACh呈内向整流和浓度依赖性。结论 :PC1 2细胞培养方便 ,同源性好 ,加入NGF后向交感神经元分化 ,且其具有神经元烟碱受体 ,可以作为交感神经元烟碱受体研究的很好的模型系统。Aim and Methods: The properties and sensitivity to acetylcholine of PC12 cells differentiated with nerve growth factor (NGF) have been investigated by using whole cell clamp technique. Results: When cultured in the presence of NGF, PC12 cells not only differentiated to resemble sympathetic neurons morphologically, but also developed electrical excitability. NGF treated PC12 cells were highly sensitive to ACh than untreated cells. The I ACh proved to be generated by nAChR by pharmacological identification. Nicotinic receptor was characterized by desensitization. The macroscopic I ACh was inward rectified and concentration dependent. Conclusion: PC12 cells are easily cultured and provides a homogenous population of cells. When culture in NGF, they differentiate to sympathetic like neurons that contain on their surface neuronal nAChR, it can be used as good model system for studying regulation of a sympathetic neuronal nAChR.

关 键 词：PC12细胞 乙酰胆碱 膜片钳技术 神经生长因子 

分 类 号：R338.8[医药卫生—人体生理学]