左旋四氢巴马汀对大鼠脑微血管内皮细胞缺氧-复氧时细胞间粘附分子-1的影响  

Effets of L-tetrahydropalmatine on ICAM-1 expression in cerebral microvascular endothelial cells during hypoxia-reoxygenation

作　　者：蒋崇慧[1] 侯德仁[2] 杨光田[3] 杨期东[2] 邓普珍[3] 

机构地区：[1]广东省中山市人民医院急诊科,528400 [2]中南大学附属湘雅医院神经内科 [3]华中科技大学附属同济医院急诊科

出　　处：《中华急诊医学杂志》2003年第1期24-26,共3页Chinese Journal of Emergency Medicine

基　　金：广东省中山市科委科研基金项目 (2 0 0 1A0 5 6)

摘　　要：目的　探讨左旋四氢巴马汀 (L -THP)对缺氧 复氧时脑微血管内皮细胞细胞间粘附分子 1(ICAM 1)表达的影响。方法　采用流式细胞仪检测脑微血管内皮细胞在缺氧、缺氧 复氧及加入L -THP后ICAM 1的阳性细胞百分率。结果　缺氧 12h ,脑微血管内皮细胞ICAM 1的表达与常氧时差异无显著意义 ,缺氧 12h再给氧 12h ,ICAM 1的表达明显增加 ,加入L -THP后 ,ICAM - 1的阳性细胞百分率明显降低。结论　L -THP可减少缺氧 复氧时脑微血管内皮细胞ICAM -Objective To investigate the effect of L tetrahydropalmatine(L THP) on the intercellular adhesion molecule-1(ICAM-1) expression in cerebral microvascular endothelial cells during hypoxia reoxygenation.Methods In cultured cerebral microvascular endothelial cclls during hypoxia reoxygenation,with and with out addition of L THP,the percentage with ICAM-1 expression was measured by flow cytometry.Results The ICAM-1 expression did not increase in cerebral microvascular endothelial cells during 12 hours of hypoxia,compared with control group,it increased after 12 hours of,and decreased after giving L-THP.Conclusion L-THP may decrease the expression of ICAM-1 in cerebral microvascular endothelial cells during hypoxia-reoxygenation. [

关 键 词：缺氧-复氧 脑微血管内皮细胞 细胞间粘附分子-1 左旋四氢巴马汀 

分 类 号：R965[医药卫生—药理学]