差谱计算分光光度法的初步研究  

A Tentative Study on Differential Computational Spectrophotometry

作　　者：陈静[1] 鄢禹平 

机构地区：[1]黑龙江中医药大学附属第二医院,哈尔滨150001 [2]哈尔滨中药四厂

出　　处：《黑龙江医药》2003年第1期23-25,共3页Heilongjiang Medicine journal

摘　　要：目的:在差谱法(差示分光光度法,吸光度减法)和多元回归分光光度法的基础上提出新的计算分光光度法—差谱计算分光光度法,并对其进行实验验证。方法:采用本文提出的方法测定复方甲硝唑片中 VB_6的含量。结果:VB_6线性范围1～10μg/mL,r=0.9999,平均回收率为98.67%,RSD=0.5%(n=6)。结论:该法适用于复方制剂中低组分含量的定量,方法简便,结果满意。OBJECTIVE:A new computional spectrophotometry—defferential computational spectrophotometry was proposed on the base of defferential spectrophotometry and multiple re- gression spectrophotometry in this article.The method was also proved by experiments.METH- ODS:The content of Vitamin B_6 in Metronidazole compound was determined with the method. RESULTS:The linearity was obtained over the range of 1～10μg/mL,r=0.9999,The average re- covery was 98.67% with RSD=0.5%(n=6).CONCLUSION:The method was simple and suit- able for quantitive analysis of the low component in compound preparations.

关 键 词：差谱计算分光光度法 多元回归分光光度法 复方甲硝唑片 

分 类 号：O657.7[理学—分析化学]