禽I型副粘病毒广西分离株F基因序列及毒力的研究  

Sequence Analysis of Fusion(F) Genes and Virulence Evaluations of GuangxiIsolates of Avian Paramyxovirus Type 1

作　　者：韦平[1] 刘禄[1] 韦天超[1] 左天荣[1] 李康然[1] 甘甲忠 

机构地区：[1]广西大学,广西南宁530005 [2]广西玉林市兽医站,广西玉林537000

出　　处：《中国预防兽医学报》2003年第2期104-106,131,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：广西自然科学基金 (桂科字 0 2 4 90 0 6 );广西玉林市科研项目 (玉市科字 [2 0 0 2 ]5 0_5 )

摘　　要：对源于鸡、鹅、鸽的 9株APMV_1广西分离株及我国常用的中发型疫苗毒株I系 (Mukteswar株 )的F基因N_端前段进行了RT_PCR扩增 ,产物进行核苷酸序列测定 ,用基因分析软件DNAStar进行分析并与已发表的其它参考毒株进行比较。结果发现 ,广西分离株在裂解位点的氨基酸组成和排列均符合强毒株的特征 ;根据F基因绘制的系谱树来看 ,广西鸡和鹅分离株都归属于基因型VII,证实近年来广西流行的基因型与国内其他地区的相同 ;并发现中发型疫苗毒株I系 (Mukteswar株 )也属于基因型VII。研究还对分离株进行了常规的毒力和致病性测定试验 ,并与根据F基因序列特征判定的结果相比较 ,结果发现其中一株鸽源和两株鹅源分离株根据常规方法判定的结果与毒株在临床上的致病性不相符 。The N_terminal nucleotides 47～420 of the Guangxi APMV_1 isolates of different poultry species origin were amplified and sequenced.The alignment and phylogenetic analysis of the nucleotide sequences and deduced amino acid sequences of F gene of the Guangxi isolates and other reference strains,obtained from GenBank,were done by using DNAStar(DNASTAR Inc.,Madison,WI).The results indicated that the component and arrangement of amino acid of all Guangxi isolates,at the cleavage site of F gene,have the typical pattern of NDV virulent strains,and were identical with the facts in the field cases.All the Guangxi isolates are classified into genotypeⅦ of APMV_1,the same genotype dominated in China and other areas in recent years.Furthermore,we also found that Mukteswar,a widely used vaccine strain in China,was classified into genotype Ⅶ.The pathogenicities of Guangxi isolates were evaluated based on the classical standards MDT and ICPI,and compared with the results based on the sequence features of cleavage site of F gene and it was found that the former was not identical with the facts in the field cases for some isolates,but the latter was fully identical.

关 键 词：禽Ⅰ型副粘病毒 广西分离株 F基因序列 毒力 研究 

分 类 号：S858.326.5[农业科学—临床兽医学] Q78[农业科学—兽医学]