脑清口服液的薄层鉴别及含量测定  

Quality Standard Establishment of Naoqing Oral Liquid

作　　者：李冬梅[1] 路绪文 许永大 苏芳[2] 

机构地区：[1]中国人民解放军第251医院,河北张家口075000 [2]中国人民解放军白求恩军医学院,河北石家庄050081

出　　处：《解放军药学学报》2003年第1期40-41,共2页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的　建立脑清口服液中葛根素的鉴别及含量测定方法。方法　采用TLC方法鉴别主要有效成分葛根素 ;HPLC法测定葛根素的含量。结果　TLC鉴定显示样品溶液在对照品溶液的相应位置处显示荧光斑点 ;HPLC法显示葛根素在浓度 2 5～ 5 0 0 (μg·ml-1)范围内与峰面积积分值呈良好的线性关系 ,加样回收率为 99.78% ,RSD 为 2 .6 2 %。结论　该法简便、准确、灵敏度高 。Aim To establish a quality standard of Naoqing oral liquid.Methods Using TLC method to justify main effective component puerarin and using HPLC method to determine the content of puerarin in Naoqing oral liquid.Results TLC showed that the fluorecent spot in the chromatogram obtained with the sample solution correspond in position to the spot in the chromatogram obtained with the reference solution.HPLC showed that there was a good linear relation when puerarin concentrations were at range of 25～500(μg·ml -1 ).Conclusion The method is simple,accurate and sensible,and can be used for quality control of this preparation.

关 键 词：薄层鉴别 含量 测定 脑清口服液 葛根素 TLC HPLC 中药 

分 类 号：R286.0[医药卫生—中药学]