3例艾滋病患者不同组织来源HIV分离株异同性的研究  

Heterogeneity of HIV strains isolated from different tissues of 3 AIDS patients

作　　者：张晨阳[1] 蒋岩[2] 邢辉[2] 冯毅[2] 潘品良[2] 范秀娟[2] 张辉[2] 刘德恭[3] 邵一鸣 

机构地区：[1]北京地坛医院,100011 [2]卫生部艾滋病预防与控制中心参比室 [3]北京佑安医院

出　　处：《中华实验和临床病毒学杂志》2003年第1期58-61,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的　了解艾滋病 (AIDS)患者外周血和部分组织人免疫缺陷病毒 (HIV)生物学性状和基因序列的特点。方法　将 3例患者的PBMC、淋巴结活检组织或脑脊液接种于PHA刺激 72h的正常人PBMC进行病毒分离 ,将分离的毒株接种于MT2细胞 ,观察分离株的致细胞病变性。同时提取前病毒DNA ,对外膜基因进行序列分析。结果　同一患者不同组织来源的毒株 ,其生物学性状和基因序列存在差异 ;2例来自脑脊液的毒株 ,其基因序列与国际标准株SF162同源性较高。结论　在同一宿主体内的不同组织、体液内有不同的病毒种群存在 ;Objective To reveal the characteristics of genotype and phenotype of HIV strains in blood and some tissues of AIDS patients.Methods The virus was isolated from peripheral blood mononuclear cell (PBMC),cerebrospinal fluid (CSF)and lymph nodes of 3 AIDS patients by coculture with PBMC stimulated by PHA for 72 hours from uninfected donor.The cytopathic effect of the HIV isolates was determined in cultured MT2 cell line.The env gene sequences form proviral DNA were analyzed by GCG software.Results In one patient,there were differences between the strains from blood and different tissues both in genotype and phenotype.The biological phenotypes of two strains from CSF were non syncytium (NSI) type, their env sequences were similar to standard CNS tropic strain (SF162).Conclusion The viral heterogeneity exists in different body compartments within an infected individual.The neurotropic isolate which is similar to international standard strain exists in some AIDS patients in China.

关 键 词：获得性免疫缺陷综合征 HIV 基因序列 细胞培养 

分 类 号：R512.91[医药卫生—内科学]