反相毛细管电色谱中中性化合物的保留及有机改性剂对其保留值的影响  

Retention of Neutral Compound in Reversed-phase Capillary Electrochromatography and Effect of Organic Modifier on It

作　　者：尤慧艳[1] 张维冰[1] 单亦初[1] 阎超[1] 张玉奎[1] 

机构地区：[1]中国科学院大连化学物理研究所国家色谱研究分析中心,辽宁大连116011

出　　处：《分析测试学报》2003年第2期5-7,共3页Journal of Instrumental Analysis

基　　金：中国国家重点基础研究发展规划(973)项目(001CB510202)

摘　　要：以硫脲作为电渗流标记物测定的死时间和以苯同系物线性回归方法测定的死时间为基础 ,将液相色谱中溶质保留值方程应用到电色谱中 ,得到了容量因子与二元流动相体系中有机改性剂含量之间的关系曲线;通过实验数据说明了一些极性的电中性化合物在电场作用下也会发生迁移而引起保留值的变化 ,且这种变化还受有机改性剂含量的影响。The dead time was obtained from thiourea and calculated from retention time of benzene homologues,and they were compared.Solute retention equation for high performance liquid chromatography was applied to capillary electrochromatography based on this.Relation curve of retention value with content of organic modifier in binary mobile phase system was obtained.The experimental results showed some polar compounds that are not charged could migrate under electric field due to their polarity and their retention value could be changed.The change was influenced by content of organic modifier in mobile phase.

关 键 词：毛细管电色谱 中性化合物 保留值 有机改性剂 

分 类 号：O657.8[理学—分析化学]