静电场对表面等离子体子共振DNA传感器灵敏度的影响  

Effects of Electrostatic Field on Senitivity of Surface Plasmon Resonance DNA Biosensor

作　　者：王青[1] 王柯敏[1] 羊小海[1] 莫远尧[1] 黄杉生[1] 李杜[1] 

机构地区：[1]湖南大学化学生物传感与计量学国家重点实验室化学化工学院,湖南长沙410082

出　　处：《湖南大学学报（自然科学版）》2003年第1期1-4,共4页Journal of Hunan University:Natural Sciences

基　　金：国家自然科学基金(29675005)及国家杰出青年基金(29875110)资助

摘　　要：利用电场改变金膜表面固定化探针DNA的方位,从而影响了DNA杂交效率,进而影响了表面等离子体子共振DNA传感器的灵敏度.结果表明不同极性的电场对DNA传感器的作用不同:当金膜表面带负电荷时,探针DNA在电场的作用下直立在金膜上,空间位阻减小,提高了杂交效率,从而使传感器的灵敏度提高,反之亦然.The combination of surface plasmon resonace (SPR) DNA biosensor with direct electroatic fie was described. In which, the hybridization efficiency was affected according to the orientation change of DNA probes caused by the eletric field, and the sensitivity of such a sensor was also changed. The results demonstrate that electric field with different poarity result in the different sensitivity of SPR DNA biosensors. When the gold film of SPR is electronegative, DNA probes immobilize on the gold surface stand straight up, therefore the hybridization effciency is increased owing to the decrease in steric hindrance, resulting in the increace of the sensitivity of SPR DNA biosensor accordingly, and vice versa.

关 键 词：表现等离子体共振 DNA 传感器 静电场 

分 类 号：Q523[生物学—生物化学]