猪瘟C-株和野毒株p80基因的序列分析  

Sequences Analysis of p80 Gene of HCV C-strain Derived from Rabbit Spleen Tissue and GXYL Strain

作　　者：庄淑珍 刘湘涛[2] 韩雪清[2] 张彦明[3] 张永国[3] 薛青红[3] 赵卫东 谢庆阁[2] 

机构地区：[1]新疆伊犁州畜牧科研所,新疆伊宁835000 [2]中国农业科学院兰州兽医研究所,甘肃兰州730046 [3]西北农林科技大学畜牧兽医学院,陕西杨凌712100

出　　处：《新疆农业大学学报》2001年第3期13-19,共7页Journal of Xinjiang Agricultural University

基　　金：国家B类攀登计划项目 (85 - 4 4 )

摘　　要：利用RT PCR及nPCR技术扩增出了C 株兔脾组织毒和广西玉林野毒 (GXYL) p80基因的一段长 94 2bp的序列 ,并将其克隆到PMD 18T载体中 ,测定其核苷酸序列并推导出了相应的氨基酸序列。比较C 株、GXYL株和已发表的Alfort株、Brescia株相应核苷酸序列及氨基酸序列同源性 ,核苷酸同源性最低为 87.4 6% ,最高为 98.0 8% ;氨基酸同源性最低为 94 .2 7% ,最高为 98.0 9%。The p80 genes of C strain derived from rabbit spleen tissue and GXYL strain were amplified by reverse transcription polymerase reaction(RT PCR) and nest polymerase reaction(nPCR) and were cloned and sequenced. Comparison on the nucleotide sequences and deduced amino acid sequences of the C strain,GXYLstrain.Alfort strain and Brescia strain showed that the homology of nucleotide sequence was from 87.46% to 98.08%, and that of the amino acid sequence A was from 94.27% to 98.09%.These prove again that the p80 gene is highly conservedin HCV.

关 键 词：猪温病毒 p80基因 C-株 序列分析 

分 类 号：S852.651[农业科学—基础兽医学]