17β-雌二醇长时程作用促进成骨细胞HOS TE85^(45)Ca摄取及间质矿化  

Long-term effects of 17β-estradiol in promoting ^(45)Ca uptake and mineralized bone-like tissue formation in human osteoblast-like cell lines TE85

作　　者：孙兰[1] 岳赟[1] 杨京[1] 刘景生[1] 

机构地区：[1]中国医学科学院,中国协和医科大学基础医学院,北京100005

出　　处：《药学学报》2003年第3期181-184,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目 (3 9970 85 6)

摘　　要：目的　研究 17β 雌二醇 (17β estradiol,E2 )对HOSTE85细胞骨形成的长时程效应。方法　3H 胸腺嘧啶参入法 (3H TdR)测细胞增殖 ;4 5Ca沉积法测细胞钙摄取 ;茜素红染色法测间质矿化。结果　E2 (0 1～ 10nmol·L- 1 )作用14d剂量依赖地刺激细胞3H TdR参入、增加4 5Ca摄取并增加茜素红染色面积。ICI1 82 ,780 (1 0nmol·L- 1 )能部分抑制E2作用 ,使其3H TdR参入分别减少了 36 5 6 % ,19 6 %和 15 4 % ,4 5Ca的摄入则分别减少了 2 2 2 7% ,37 2 8%和 4 0 1% ,茜素红染色面积减少。结论　E2 通过增加细胞数量。Aim To study the long term effects of 17β estradiol (E 2) on cell proliferation, 45 Ca up take, and mineralized bone like tissue formation in human osteoblast like cell line TE85. Methods Human osteoblast like cell line TE85 was used as osteoblast cell model. Using methods of 3H thymidine incorporation for cell proliferation and 45 Ca deposit for calcium uptake, and Alizarin red S dye for mineralized bone like tissue. Results Compared with the cells of control group, 3H thymidine incorporation into TE85 cells was significantly increased (85 65%, 93 42% and 106 58%, respectively) and 45 Ca uptake was increased (101 35%, 130 9% and 169 5% respectively), after treated with E 2 (0 1, 1 0, 10 nmol·L -1 ) for 14 days. The stained area of Alizarin red S in E 2 treated cells was also increased obviously. ICI 182,780 , a specific antagonist of estrogen receptor, was shown to partly inhibit E 2 induced actions with the inhibition ratio of 19 6% or 37 28% in both experiments of 3H TdR and 45 Ca uptake on the condition of E 2 (1 0 nmol·L -1 ). Conclusion E 2 was found to increase the mineralized bone like tissue by enhancement of cell proliferation and promotion of calcium uptake, which were in favor of bone formation. These actions may be partly mediated by estrogen receptor.

关 键 词：17Β-雌二醇 成骨细胞 ^3H-胸腺嘧啶参入法 钙摄取 矿化骨样组织 

分 类 号：R963[医药卫生—微生物与生化药学] R977.1[医药卫生—药理学]