应用流式细胞仪研究Pb对海洋微藻生长的影响  

The influence of lead on marine microalgae by a flow cytometry

作　　者：何学佳[1] 彭兴跃[1] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005

出　　处：《海洋环境科学》2003年第1期1-5,共5页Marine Environmental Science

基　　金：国家自然科学基金资助项目(49806004);高等学校骨干教师资助计划项目

摘　　要：运用流式细胞仪研究比较了Pb对3种常见海洋单细胞微藻:湛江叉鞭金藻(Dicrateriazhanjiangen sis)、球等鞭金藻(Isochrysisgalbana)、小球藻(Chlorellasp.)的毒害作用。用内插法求得Pb对3种微藻的96h的半有效浓度(96hEC50)分别为9.03、8.83和>20.00mg/L。Pb抑制细胞的生长分裂,使延滞期延长,并影响了叶绿素正常合成,毒性与浓度成正相关。Pb对不同生长期的细胞的作用强度不同,对生长分裂和叶绿素合成的作用程度呈现差异性。The effects of lead on the division and chlorophyll synthesis of Dicrateria zhanjiangensis, Isochrysis galbana and Chlorella sp. were studied using a flow cytometer. The results showed that the 96h·EC values of these three species of algae were 9.03 mg/L, 8.83 mg/L and >20.00 mg/L respectively. Lead inhibits the cell division, lengthened lag phase and caused abnormality of chlorophyll synthesis. Its toxity is increased as lead concentration increased. For any of the three species studied, the impacts of Lead on the cells of the two different phases of growth differed. It was also suggested that inhibition of chlorophyll synthesis was relatively insensitive to lead compared with that of the cell division rate. 

关 键 词：微藻 流式细胞仪 PB 细胞生长分裂 叶绿素 

分 类 号：X503.2[环境科学与工程—环境工程] Q178.53[生物学—水生生物学]