开心胶囊对糖尿病大鼠心肌p53 mRNA表达的调节  

Effects of Kaixin capsule(开心胶囊) on p53 mRNA gene expression of myocardium in diabetic rats

作　　者：罗小星[1] 靳利利[1] 薛军[1] 陈镜合[1] 

机构地区：[1]广州中医药大学第一附属医院ICU,广东广州510405

出　　处：《中国中西医结合急救杂志》2003年第2期114-115,共2页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

摘　　要：目的 :探讨糖尿病大鼠心肌细胞 p5 3 m RNA表达和开心胶囊的影响。方法 :建立大鼠链尿佐菌素诱导的糖尿病动物模型 ,设立对照组、非治疗组及开心胶囊组 ,用逆转录聚合酶链式反应检测病程 4周和 8周大鼠心肌细胞 p5 3水平。结果 :诱发糖尿病 4周后糖尿病大鼠心肌细胞 p5 3m RNA未见明显表达增强 ,8周时表达较明显 ( P<0 .0 5 ) ;给开心胶囊 8周后 ,p5 3m RNA表达明显下降 ( P<0 .0 5 )。结论 :糖尿病状态下 ,心肌细胞p5 3m RNA高表达可能是高血糖诱发结果 ,开心胶囊可通过减轻 p5 3m RNA异常表达缓解糖尿病心肌损伤。Objective:To study the effects of Kaixin capsule(开心胶囊) on p53 mRNA gene expression of myocardium in diabetic rats.Methods:Diabetic rat model was established by steptozolin induced,and then randomly divided into control group,nontreated group,and Kaixin capsule treatment group.The reverse transcriptionpolymerase chain reaction (RTPCR) was done for determining the expression level of p53 mRNA in the rats with diabetes for 4 weeks and 8 weeks. Results:p53 mRNA expression in 8 weeks diabetic duration was significantly enhanced (P<0.05),but it was not found in 4 weeks.Administration of Kaixin capsule had a significant remission after its administration for 8 weeks (P<0.05).Conclusion:The enhanced expression of myocardial p53 mRNA may result from hyperglycemia in the diabetic state.Kaixin capsule may alleviate diabetic injury to myocardium through modifying the abnormally enhanced expression of p53 mRNA.

关 键 词：糖尿病 开心胶囊 53mRNA 逆转录聚合酶链式反应 

分 类 号：R285.5[医药卫生—中药学]