麻黄汤合煎汤剂与颗粒汤剂中苦杏仁苷含量测定  

Determination of Amygdaloside in Ephedra Decoctions by HPLC

作　　者：梁光义[1] 曹佩雪[1] 李霞 周飞 柴立 

机构地区：[1]贵州省,中国科学院天然产物化学重点实验室,贵州贵阳550002 [2]贵州宏宇药业有限公司,贵州贵阳550002

出　　处：《现代中药研究与实践》2003年第1期30-32,共3页Research and Practice on Chinese Medicines

基　　金：国家自然科学基金资助项目 ( 3 9960 0 83 )

摘　　要：目的　比较用合煎方法及用颗粒沸水冲泡法制备的麻黄汤中苦杏仁苷的含量变化。方法　采用反相高效液相色谱法。以 Hypersil C1 8柱为分析柱 ,以甲醇 -水为流动相作梯度洗脱。结果　苦杏仁苷峰能与其它峰有效分离。同时测得颗粒冲泡法制备的麻黄汤苦杏仁苷的含量高于合煎汤剂。结论　用颗粒冲泡法制备麻黄汤 ,苦杏仁苷的含量较高 。Objective To compare the contents of amygdaloside in Ephedra Decoctions made by using traditional method with that by soaking granules with boiling water.Methods The samples were determined by HPLC.Using C 18 column with methanol water as mobile phase and detection wavelength at 215 nm.Results This method shows a good separating degree.The content of amygdaloside in granule decoction is higher than that in traditional decoction.Conclusion It can be used in clinic as a reference.

关 键 词：麻黄汤 苦杏仁苷 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学]