卡氏肺孢子虫在小鼠肺内的发育及引起的病理变化  

Pathological Changes Induced by the Growth of Pneumocystis CariniiIn Mice Lungs

作　　者：郭鄂平[1] 唐慕湘[2] 宋明华[1] 

机构地区：[1]郧阳医学院寄生虫学教研室 [2]郧阳医学院病理学教研室,湖北十堰442000

出　　处：《医学动物防制》2003年第2期81-84,共4页Journal of Medical Pest Control

摘　　要：目的　观察小鼠卡氏肺孢子虫肺炎病原和病理学改变。方法 :昆明小鼠皮下注射醋酸可的松诱发小鼠卡氏肺孢子虫肺炎。肺印片 Giem sa染色检查卡氏肺孢子虫包囊 ;常规石蜡切片 ,HE染色、PAS染色及 GMS染色观察病理学改变。结果 :自用药第 5周肺印片即可检出卡氏肺孢子虫包囊 ,第 8周包囊检出率达 81.82 % ;HE染色切片早期以淋巴细胞、单核细胞渗出为主的间质性肺炎 ;晚期间质性肺炎加重 ,肺泡腔内出现泡沫样渗出物 ;PAS染色切片泡沫样渗出物呈桃红色阳性反应 ;GMS染色切片可见染成黑色的包囊。结论 :本研究为建立卡氏肺孢子虫肺炎动物模型提供了病原形态学和病理学依据。Objective: To observe pathogenic and Pathologica l changes of pneumocystis carinii pneumonia in mice. Methods: Ku nming mice underwent subcutaneous injection of cortisone acetate so that pneumoc ystis carinii pneumonia was induced. Form the pulmonary impression by Giemsa's s taining ,the cysts and trophozoites of pneumocystis carinii were found. Histolog ical changes were studied on the usual paraffin section by three kinds of staini ng, including HE, PAS and GMS.Results:5weeks after the administrat ion of drug, the cysts of pneumocystis carinii could be identified in the pulmon ary impression. 7week later, the detectable rate was 81.82%. The histological ch anges included: early interstitial pneumonitis with infiltration of lymphocytes and monocytes, later interstitial pneumonitis being worse, and identification of foamy intra-alveolar exudates. With PAS staining, the exudates showed pink posi tive reaction. Black cysts were found in GMS staining section. Conclusion : The study would give pathogenic and pathological evidence for developin g the animal model of pneumocystis carinii pneumonia.patho

关 键 词：卡氏肺孢子虫 卡氏肺孢子虫肺炎 病原形态学 病理学 动物实验 

分 类 号：R531.5[医药卫生—内科学]