报告基因显像监测基因治疗研究进展  

Advances in study of reporter gene imaging for monitoring gene therapy

作　　者：穆传杰[1] 周继文[1] 

机构地区：[1]中国医学科学院

出　　处：《国外医学（放射医学核医学分册）》2003年第1期4-8,共5页Foreign Medical Sciences(Section of Radiation Medicine and Nuclear Medicine)

摘　　要：为评价基因治疗,需要随时对治疗基因的定位和表达进行监测。放射性核素报告基因技术是检测基因表达的最好方法。用基因融合、双顺反子、双启动子及双向转录等重组技术,构建表达报告基因的腺病毒载体,导入靶细胞或组织内,然后注射与报告基因偶合的核素标记的探针,进行PET、SPECT或γ-照相,可无创伤地、重复地定量显示报告基因表达。目前,用于基因治疗的报告基因和报告探针系统有:HSV1-tk(单纯疱疹病毒胸腺嘧啶核苷激酶基因)和碘、氟同位素标记的尿嘧啶、鸟嘌呤的衍生物;突变的多巴胺D2R(多巴胺2型受体)基因和18F-FESP(18F-氟乙基螺环哌丁苯);SSTr2(生长抑素2型受体基因)和生长抑素类似物等。其中,部分已用于临床试验治疗。To evaluate the efficiency of gene therapy,it is requisite to monitor localization and expression of the therapeutic gene in vivo.Monitoring expression of reporter gene using radionuclide reporter gene technique is the best method.Adenoviral vectors expressing reporter gene are constructed using gene fusion,bicistronic,double promoter or bidirectional transcriptional recombination techniques,and transferred into target cells and tissues,then injected radiolabeled reporter probes which couple to the reporter genes.The reporter genes can be imaged invasively,repeatedly,quantitatively withγ-camera,PET and SPECT.Recently,several reporter gene and reporter probe systems have been used in studies of gene therapy.The part of them has been used for clinic trials.

关 键 词：报告基因 报告探针 基因显像 基因治疗 

分 类 号：R456[医药卫生—治疗学]