愈肝片对D-氨基半乳糖小鼠急性肝损伤的保护作用  

Protective Effect of Yugan Tablet on D - galactosamine - induced Acute Hepatic Injury in Mice

作　　者：刘立昌[1] 张磊[2] 邓毅峰[3] 周鸣[3] 张永和[3] 

机构地区：[1]深圳市第二人民医院,深圳518035 [2]湖北中医学院2000级研究生,武汉430061 [3]长春中医学院,长春130021

出　　处：《广州中医药大学学报》2003年第1期57-58,62,共3页Journal of Guangzhou University of Traditional Chinese Medicine

摘　　要：[目的]观察愈肝片(由生晒参、枸杞子、丹参、土茯苓、柴胡、郁金、石见穿等中药制成)对D-氨基半乳糖胺(D—GlaN)小鼠急性肝损伤的保护作用。[方法]将60只小鼠随机分为空白对照组、模型组、肝脾康阳性对照组和愈肝片高、中、低剂量组,每组10只。测定血清白蛋白(ALB)和天门冬氨酸氨基转移酶(AST)和凝血时间(CT),观察了愈肝片和阳性对照组对D—GlaN肝损伤模型的影响。[结果]与空白对照组比较,模型组小鼠血清天门冬氨酸氨基转移酶(AST)含量升高(|P<0.01),血清白蛋白(ALB)含量降低(P<0.01),凝血时间延长(P<0.01),表明模型组动物肝细胞受到损伤,而愈肝片高剂量组动物的AST含量降低(与模型纽比较P<0.05)。同时,愈肝片各剂量组动物血清白蛋白含量比模型组升高(P<0.05或P<0.01);愈肝片的中、高剂量组对动物凝血时间延长有改善作用(与模型组比,P<0.05)。[结论]愈肝片对D—GlaN所致的肝损伤有一定的保护作用。[Objective] To observe the protective effect of Yugan Tablet (YT) on D-galactosamme-induced acute hepatic injury in mice. [ Methods ] Mice were randomly allocated to normal control group, model group, Ganpi Kang Capsule group and YT groups (low-, moderate- and high-dosage YT). Serum levels of aspartate transaminase (AST) and albumin (ALB) and clotting time (CT) were examined to evaluate the effects of YT and Ganpi Kang Capsule on mice with D-galactosamine-induced acute hepatic injury. [Results] Serum level of AST was increased ( P < 0.01) , ALB level decreased ( P < 0.01) and CT prolonged (P<0.01) in model mice as compared with those in normal control group, which indicated that hepatic cells in model mice was injured. After treatment, AST level was decreased in high-dosage YT (P < 0.05), ALB increased in the three YT groups ( P < 0.05 or P < 0.01) and CT shortened in moderate- and high-dosage YT groups ( P < 0.05) as compared with those in model group. [Conclusion] YT has a certain protective effect on D-galactosamine-induced acute hepatic injury in mice.

关 键 词：愈肝片 药理学 肝损伤 中药疗法 天冬氨酸转氨酶 血液 白蛋白类 疾病模型 动物 小鼠 

分 类 号：R285.5[医药卫生—中药学] R269.573[医药卫生—中医学]