SPE-HPLC法测定六味地黄丸中丹皮酚的含量  

Content Determination of Paeonol in Liuwei Dihuang Pill by SPE - HPLC

作　　者：李玮玲[1] 林生文[2] 胡育筑[1] 

机构地区：[1]中国药科大学,南京210038 [2]广东省药品检验所,广州510180

出　　处：《中药新药与临床药理》2003年第2期116-119,共4页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的 采用SPE—HPLC法测定六味地黄丸中丹皮酚的含量。方法 以甲醇-2％冰醋酸(55:45)为流动相,Hypersil ODS(2)柱(250 mm×4.6 mm,5 μm)为固定相,流速为1 mL/min,检测波长为275 nm。结果 丹皮酚的测定在0.03998～0.7996 μg范围内线性关系良好,大蜜丸的平均回收率为102.9％,RSD为1.2％;浓缩丸的平均回收率为101.5％,RSD为1.8％。结论 该方法简单、灵敏度高,可用于六味地黄丸中丹皮酚的含量测定。Objective To develop SPE - HPLC method for the assay of paeonol in Liuwei Dihuang Pill (LDP). Methods Separation was performed on a Hypersil ODS(2) column (250 mm×4. 6 mm, 5μm) . The mobile phase consist of methanol - 2 % acetic acid (55 :45, v/v) . The flow rate was 1 mL/min. The UV detection was set at 275 nm. Results Paeonol had a good linear relation in the range of 0. 03998 ~ 0. 7996 ug, the average recovery of honeyed bolus of LDP was 102. 9 % and the RSD was 1.2%; the average recovery of concentrated bolus of LDP was 101. 5 % and the RSD was 1.8%. Conclusion The method is simple, sensitive and rapid and it can be used to determine the paeonol content of LDP.

关 键 词：六味地黄丸 化学分析 丹皮酚 固相萃取 高压液相色谱法 

分 类 号：R284.1[医药卫生—中药学]