麝香酮抗局灶性脑缺血损伤的实验研究  

Study of protection of muscone against local cerebral ischemia

作　　者：梁辉[1] 陈虎[1] 高颖[1] 蔡定芳[1] 

机构地区：[1]复旦大学附属华山医院中西医结合研究所,上海200040

出　　处：《中成药》2003年第3期225-227,共3页Chinese Traditional Patent Medicine

基　　金：上海市百人计划

摘　　要：目的 :研究麝香酮对局灶性脑缺血模型大鼠梗塞体积及缺血海马神经元谷氨酸转运体EAAC1mRAN表达的影响。方法 :实验分正常组、模型组、药物组 ,用插线法建立大鼠局灶性脑缺血模型 ,造模前半小时予灌服麝香酮 ,采用四氮唑红(TTC)染色和半定量逆转录 聚合酶链式反应 (RT PCR)法 ,测定麝香酮对梗塞体积和海马EAAClmRNA水平的变化。结果 :麝香酮可缩小缺血大鼠梗塞体积 ,有效下调EAAClmRNA表达。结论 :麝香酮具有抗脑缺血损伤作用 。Objective: To investigate the effect of muscone on infarction volume and neuronal glutamate transporter EAAC1 mRNA expression in rats. Methods: Rats were divided randomly, focal ischemic models of middle cerebral artery occlusion (MCAO) were achieved by inserting nylon surgical thread through the internal carotid artery(ICA) into the anterior cerebral artery(ACA). The rats were sacrificed, the brain was stained by TTC and the area of infarcton was measured. The expression of EAACl mRNA in the hippocampal regions was assessed by RT PCR. Results: Muscone could remarkably reduce tbe infarction volume and expression of EAAC1 mRNA. Conclusion: Muscone plays a significant role in against local cerebral ischemia, the mechanism may be related to reducing EEACl mRNA expression.

关 键 词：局灶性脑缺血 神经元保护 神经元谷氨酸转运体 麝香酮 

分 类 号：R285.5[医药卫生—中药学] R277.733.1[医药卫生—中医学]