一氧化氮合酶抑制剂对关节软骨缺损修复影响的形态学研究  

Morphological study of the effects of nitric oxide synthase inhibitor on repair of articular cartilage defects

作　　者：孙炜[1] 王吉兴[1] 刘晓霞 冯岚[1] 秦立赟 金大地[1] 

机构地区：[1]第一军医大学附属南方医院骨科,广东广州510515 [2]广东消化研究所病理研究室,广东广州510515 [3]广州军区188医院外科,广东潮州521000

出　　处：《临床骨科杂志》2003年第1期8-11,共4页Journal of Clinical Orthopaedics

基　　金：国家自然科学基金资助项目 (编号 :3 990 0 14 9)

摘　　要：目的　探讨诱导型一氧化氮合酶 (iNOS)抑制剂S 甲基异硫脲 (SMT)对关节软骨修复的影响。方法　将 2 0只新西兰大白兔双侧股骨髁关节面造成全层软骨缺损。随机分为 2组 :对照组 10例 ,缺损软骨面用纤维蛋白凝胶BMP复合物充填 ;给药组 10例 ,缺损软骨面用纤维蛋白凝胶BMP复合物充填后 ,皮下注射SMT(5mg·kg-1·12h-1)。术后 8周、16周、1年处死动物 ,按组织形态学分级标准 ,双盲法行 16周和 1年修复组织评价 ;化学比色法检测修复组织NO释放量和NOS活性。结果　形态学观察证实 ,术后 8周、16周及 1年后 ,给药组软骨缺损修复在缺损区结构、细胞形态和基质染色等评分方面优于对照组 (P <0 0 5 )。术后 16周及 1年对照组NO释放量和NOS活性明显高于给药组 (P <0 0 5 )。结论　iNOS抑制剂SMT可减少NO释放 ,降低iNOS活性 ,提高软骨修复质量。Objective To investigate the effect of nitric oxide(NO) synthase inhibitor on reparation of articular cartilage defects. Methods Full thickness defects of cartilage were created in the bilateral femoral intercondylar groove of 20 adult New Zealand white rabbits. Twenty defects were filled with a Fibrin Glue impregnated with rhBMP as the control, and the other 20 defects were filled with a Fibrin Glue impregnated with rhBMP and hypodermicly injected with 5 mg·kg -1 ·12 h -1 S Methylisothiurea(SMT). The animals were killed after eight, sixteen weeks and one year postoperatively, and the gross appearance of healed defect was assessed. The repaired tissues were observed histologically and evaluated according to a grading scale. The amount of released NO and the activity of NOS were examinated by chemical colorimetry. Results For the group of eight weeks?Sixteen weeks and one year after Fibrin Glue with rhBMP and with hypodermic injection of SMT, the defects had a better histological appearance than that of control group. The histological features that showed improvement were integration at the margin, cellular morphology, and architecture within the defect. The total scores of the defects treated with SMT were also better than that of the control( P <0 05). NO release and NOS activity in defects of the control after sixteen weeks and one year were more than that of the defects treated with SMT( P <0 05). Conclusion iNOS inhibitor SMT could decrease NO release and iNOS activity, so that the quality of tissues repair could be improved.

关 键 词：一氧化氮合酶抑制剂 S-甲基异硫脲 软骨缺损 

分 类 号：R977.3[医药卫生—药品] R684[医药卫生—药学]