IL-7对PHA-抗CD3 LAK细胞抑制效应的研究  

Depressive role of IL-7 in LAK Cell Induction from Human Mononuclear Cell Combining with IL-2,PHA and αCD3

作　　者：叶萍[1] 程一櫂[2] 王艳[2] 范亚欣[2] 郭连英[2] 钱振超[2] 

机构地区：[1]大连市红十字血液中心,大连116011 [2]大连医科大学,大连116027

出　　处：《上海免疫学杂志》2003年第2期108-110,127,共4页Shanghai Journal of Immunology

基　　金：国家"八五"攻关资助项目 (No 85 914 0 2 0 8)

摘　　要：文章将IL 7与IL 2 ,PHA及αCD3联用诱生PHA αCD3LAK细胞 ,应用活细胞计数、MTT和APAAP等方法测定了细胞增殖活性、mIL 2R的表达、外源性IL 2的消耗、细胞毒活性和效应细胞表型等指标 ,并分析比较了IL 7的加入对以上指标的影响。结果表明IL 7的加入使PHA αCD3LAK细胞消耗IL 2的量、mIL 2R表达的量和细胞的增殖能力降低 ,细胞毒活性减弱 ,并伴有CD8+ 细胞百分率的下降。这一结果呈现出的IL 7的抑制作用尚未见报道 ,有进一步研究的价值。In this paper,we induced PHA αCD3 LAK cell with IL 7,detected and compared their proliferative activity,expression of mIL 2R,consumption of exogenous IL 2,cytotoxicity and the phenotypes of effector cells with vital stain cell counting,MTT and APAAP methods in order to find the role of IL 7 on it The results indicated that in the presence of IL 2,IL 7 along with PHA priming and αCD3 costimulation showed depressive effect on PHA αCD3LAK cell In the presence of IL 7,consumption of IL 2,expression of mIL 2R and proliferative activity cut down,and cytotoxicity was weakened,in accordance with the decrease of CD8 + cell percentage As the inhibitory effect of IL 7 to PHA αCD3 LAK cell has not been reported,yet It would be worthy of further investigation

关 键 词：白细胞介素7 抗CD3单克隆抗体 淋巴因子激活的杀伤细胞 

分 类 号：R392.12[医药卫生—免疫学] R730.51[医药卫生—基础医学]