SLE患者T细胞TCR/CD3复合物介导的信号转导研究  

Study on TCR/CD3 Complex-Mediated Signal Transduction in T Cells from Patients with Systemic Lupus Erythematosus

作　　者：杨庆永[1] 廖元兴[1] 王宗发[2] 杨慧兰[1] 

机构地区：[1]广州军区广州总医院皮肤科,广州510010 [2]第一军医大学南方医院皮肤科,广州510515

出　　处：《上海免疫学杂志》2003年第2期123-125,共3页Shanghai Journal of Immunology

摘　　要：为证明SLE患者T细胞功能异常是否与其生物化学信号转导异常有关。用CD3单抗与羊抗鼠二抗IgG相交联刺激T细胞并用Thapsigargin和EGTA干预后 ,用分别粘附细胞仪连续观察 10minT细胞 [Ca2 + ]i的变化 ,并评价 [Ca2 + ]i反应与CD3分子、InsP3 生成量和患者临床特征的相关性。结果显示 :正常人和SLE患者T细胞 [Ca2 + ]i反应的基准值相似 (P =0 10 5 ) ;SLE患者高峰值、平台值T细胞的 [Ca2 + ]i反应明显高于正常对照 (P <0 0 0 1,P <0 0 0 1) ;加入Thapsigargin后二者 [Ca2 + ]i反应无显著差异 ,而加入EGTA后二者 [Ca2 + ]i反应有显著差异 ;二者的T细胞CD3阳性率和InsP3 生成量无差异 (P =0 6 6 5 ,P =0 5 37) ;且 [Ca2 + ]i异常反应与患者的疾病活动性无关。这些结果提示 :SLE患者T细胞TCR/CD3介导的信号转导途径存在异常 ;SLE患者T细胞功能异常可能是因细胞内生物化学信号途径异常所致。This article is to demonstrate whether SLE T cells function disordor is relative to abnormal biochemical pathways After cross linking of anti CD3 mAb to sheep anti mouse IgG and stimulating T cells,the changes of free calcium ion within T cells and these changes under interference of Thapsigargin and EGTA were observed respectively successively for 10 minutes by an adhesion cytometry The relation between [Ca 2+ ]i responses in SLE T cells and expression of CD3 molecule,InsP 3 levels or disease activity of SLE patients was evaluated Experimental results showed that base [Ca 2+ ]i response in T cells from SLE patients was similar to that from normal control ( P =0 105),peak and plateau [Ca 2+ ]i responses were significantly higher in the group of SLE patients ( P <0 001, P <0 001),[Ca 2+ ]i responses were similar in both individuals after addition of Thapsigargin,[Ca 2+ ]i responses of T cells from SLE patients in the presence of EGTA were higher,the percentage of the CD3 + cells and InsP 3 generation were similar in both individuals ( P =0 665, P =0 537),and the abnormal [Ca 2+ ]i response was not relative to disease activity of SLE patients These results suggested that TCR/CD3 mediated [Ca 2+ ]i responses in T cells from SLE patients was abnormal,and T cell function disorder existing in SLE was the by product of abnormal biochemical pathways

关 键 词：系统性红斑狼疮 T细胞 钙离子 TCR/CD3复合物 信号转导 

分 类 号：R593.241[医药卫生—内科学]