氯胺酮对脑冷冻伤后海马区神经细胞及其行为学的影响  

The Effects of Ketamine on Neuron in Hippocampus Region and Neurological Outcome after Cold-induced Injury

作　　者：魏兴[1] 张志琳[1] 谢红[1] 范圣登[1] 

机构地区：[1]苏州大学附属第二医院麻醉科,苏州215004

出　　处：《苏州大学学报（医学版）》2003年第1期19-21,共3页Suzhou University Journal of Medical Science

摘　　要：目的　观察脑冷冻伤后海马区神经细胞存活数和神经创伤评分 (NSS) ,并探讨N -甲基 -D -天门冬氨酸 (NMDA)受体非竞争性拮抗剂氯胺酮对神经细胞和行为学的影响。方法　 49只SD大鼠随机分为假手术组、未治疗组和氯胺酮治疗组。分别于冷冻伤后 1、2、6、2 4h进行NSS评分 ,冷冻伤后 2、6、2 4h处死动物 ,取脑作病理切片。结果　冷冻伤后未治疗组海马区神经元存活细胞数明显减少 ,氯胺酮能显著增加存活神经元细胞数 ,2 4hNSS评分明显低于未治疗组。结论　氯胺酮能减少冷冻伤后海马区神经元的丧失 ,有利于颅脑损伤后神经功能的恢复 。Objective To observe the change of neurons in hippocampus region in the rat brain and neurological severity score (NSS),also to study the effects of treatment with ketamine on neuron in hippocampus region and NSS after cold induced injury.Methods Forty nine Sprague Dawley rats were divided into three groups:sham operated group,no treatment group and ketamine treatment group.NSS was determined at 1,2,6and 24 hours after head trauma.After death at 2,6 and 24 hours,Brain was taken for pathological examining.Results Number of neurons in hippocampus region decreased after head trauma.In groups with ketamine treatment number of neurons in hippocampus region increased after trauma and NSS was lower than that in the controls markedly at 24 hours.Conclusion We conclude that 180mg/kg IP ketamine is effective in ameliorating neurological dysfunction and in improving neuron surviving after head cold injury.

关 键 词：冷冻伤 氯胺酮 NMDA受体 神经毒性 神经保护 

分 类 号：R645[医药卫生—外科学] R651.15[医药卫生—临床医学]