两种鸡大肠杆菌油佐剂灭活苗对雏鸡免疫效果的比较  

Comparison Immunization Effect of the Two Kinds Emulsion Inactivated Vaccine of E. Coli in Chicken

作　　者：党如意[1] 张淑霞[1] 杨增岐[1] 赵余放[1] 

机构地区：[1]西北农林科技大学畜牧兽医学院,陕西杨凌712100

出　　处：《西北农业学报》2003年第1期111-113,共3页Acta Agriculturae Boreali-occidentalis Sinica

摘　　要：为了探讨用鸡胚和普通培养基增殖的大肠杆菌在免疫效果方面有无差异,用致病性大肠杆菌地方菌株与标准菌株混合,经鸡胚及固体培养基增殖后,制成两种油佐剂灭活疫苗,分别免疫10日龄雏鸡,免疫后21d抗体达到峰值,抗体在3log2以上维持35d以上,免疫后42d用混合菌液对两种菌苗免疫组分别进行攻击,鸡胚苗免疫组对混合菌液的攻击保护率为93.3%,常规培养苗免疫组对混合菌液的攻击保护率为83.3%。In order to explore the differences between two vaccines used in the study, The two kinds of oil emulsion inactivated vaccine were made, in one of which, the E·coli standard strains and local strains were propagated on solid media, and in another, the same strains were propagated in embryonated eggs. 10 days chickens were vaccinated with two kinds vaccine respectively. The antibodies titer reached peaks at 21 d postimmunization and retained over 3log2 35 d. The challenge mixture bacteria acted at 42 d postimmunization. The embryonated eggs vaccine vaccinated group chickens showed 93.3% protectivity, but solid culture medium vaccine only 83.3%.

关 键 词：鸡大肠杆菌 油佐剂灭活苗 免疫 

分 类 号：S852.52[农业科学—基础兽医学]