塑料半薄切片法观察豆科植物根表面传递细胞  

Observing the Transfer Cells in the Epidermal Cells of Rootsof Leguminous Plants with Semi-thin Sections

作　　者：吴均章[1] 韩素芬[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2003年第2期65-68,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家自然科学基金资助项目(39970601)

摘　　要：豆科植物合欢、紫云英、苜蓿等在接种根瘤菌后,部分根毛密集生长稍有膨大的根段,经FAA固定,GMA包埋,半薄切片(3μm),PAS反应染色,在光学显微镜油镜下观察根的横切面发现,其表皮细胞或外皮层细胞的外切向壁上有明显的壁内突结构,属典型的根表面传递细胞。采用半薄切片,不仅操作简单,费用较少,且较易确定传递细胞发生的部位。For observing the transfer cells in epiderm of the roots by optical microscope,the method is taken as follow:after the leguminous plants Albizia julibrissin,Astragalus sinicus,Medicago sativa induced by Rhizobia,their root segments where root hair grows densely and swells a little are selected to be fixed in FAA.Through a series of treatment such as being embedded in GMA,semithin sections(3 μm),staining with PAS reaction,the root cross sections were observed by optical microscope under the oil objective.The result shows that on the tangential exowall of the epidermal cells or exodermis of the roots,the wall ingrowth is easy to be found.It is the typical epidermal transfer cells of root.This method is not only simple with a low cost but also easy to determine the position of the transfer cells in the root.

关 键 词：传递细胞 GMA 半薄切片 油镜 

分 类 号：S188[农业科学—农业基础科学]