沙眼衣原体相关性不孕患者体内抗体及细胞因子的测定  

Detection of antibodies and cytokines in women with infertility associated with chlamydia trachomatis

作　　者：王慧[1] 熊礼宽[2] 袁凤娣[3] 朱锦明[4] 

机构地区：[1]徐州医学院微生物与免疫学教研室,江苏徐州221002 [2]深圳市慢性病防治院,广东深圳518020 [3]宜兴市人民医院妇产科,江苏宜兴216000 [4]徐州市妇幼保健院妇产科,江苏徐州221009

出　　处：《徐州医学院学报》2003年第2期103-107,共5页Acta Academiae Medicinae Xuzhou

基　　金：江苏省教育厅资助项目 (0 0KJD31 0 0 0 3)

摘　　要：目的　探讨沙眼衣原体 (CT)相关性不孕患者体内沙眼衣原体热休克蛋白 (C -hsp60 )抗体、抗精子抗体(ASAb)、白细胞介素 1 (IL - 1 )、白细胞介素 6(IL - 6)、白细胞介素 8(IL - 8)、肿瘤坏死因子α(TNF -α)和γ -干扰素 (IFN -γ)的水平及作用。方法　采用PCR技术对患者宫颈分泌物进行CT检测 ,MIF测定抗CT主要外膜蛋白(MOMP)抗体IgG滴度 ,Westernblot测定C -hsp60抗体的存在以及ELISA测定血清中IgG型ASAb及输卵管液中IL - 1、IL - 6、IL - 8、TNF -α、IFN -γ的含量。结果　CTDNA阳性者 68例 ,其中 57例 (83 .8% )出现C -hsp60抗体 ,CTDNA阴性者 1 72例 ,其中 64例 (37.2 % )亦出现此抗体。不孕组 (A +B)ASAb阳性率与对照组比较 ,差异有显著性 (P <0 .0 1 ) ,不孕A组与B组比较 ,差异无显著性 (χ2 =1 .2 2 ,P >0 .0 5)。不孕A组输卵管液中IL - 1、IL- 6、IL - 8、TNF -α、IFN -γ的含量分别为 (1 1 83± 2 0 4 )ng L、(70 6± 60 2 )ng L、(787± 40 3)ng L、(1 82± 44)ng L、(2 2 1± 45)ng L ,高于对照组 (556± 1 1 2 )ng L、(2 31± 1 67)ng L、(2 38± 99)ng L、(1 2 6± 37)ng L、(1 99± 69)ng L(P均 <0 .0 1 )。结论　宫颈分泌物CT检测阴性者不能排除输卵管、盆腔等深部CT感染 ,C -hsp60抗?Objective To determine if C-hsp60 antibody, anti-sperm antibody (ASAb) and cytokines are produced in response to infertility associated with chlamydia trachomatis (CT). Methods The endocervical specimens were assayed for CT by PCR. The sera were assayed for C-hsp60 antibody by Western blot, and for ASAb, IL-1, IL-6, IL-8, TNF-α and IFN-γ by ELISA. Results The positive rate of C-hsp60 antibody was 57/68 (83.8%) in the group of CT-DNA positive patients, and was 64/172 (37.2%) in CT-DNA negative patients. Compared with that of the fertile control group, the positive rate of ASAb was significantly higher in the infertile group (P<0.01), but no significant difference was found between the infertile groups A and B (P>0.05). The mean values of IL-1, IL-6, IL-8, TNF-α and IFN-γ were (1183±204) ng/L, (706±602) ng/L, (787±403) ng/L, (182±44) ng/L and (221±45) ng/L respectively in infertile group A, all higher than those in infertile group B (P<0.05). Conclusion A negative PCR result of CT-DNA for endocervical specimen can not rule out possible salpingian and pelvic chlamydial infections, while the detection of C-hsp60 antibody does help diagnose the infection of deeper genital tract. The levels of IL-1, IL-6, IL-8, TNF-α and IFN-γ will be elevated along with salpingian chlamydial infection, indicating that a delayed hypersensitivity may be responsible for the infertility associated with CT, and that the C-hsp60 antibody and cytokines mentioned above may play important roles in the pathogenesis.

关 键 词：沙眼衣原体 抗体 不孕 白细胞介素1 白细胞介素6 白细胞介素8 肿瘤坏死因子α Γ-干扰素 

分 类 号：R392.11[医药卫生—免疫学]