联合应用特乐定与固邦对去卵巢大鼠骨骼作用研究  

Effects of l-glutamine monofluorophosphate and alendronate sodium on trabecular bone in ovariectomized rats

作　　者：陈建波[1] 李青南[2] 黄连芳[2] 胡彬[2] 陈艳[2] 

机构地区：[1]广州医学院医学检验中心,广州510182 [2]广东医学院骨生物学研究室,湛江524023

出　　处：《中国药理学通报》2003年第3期326-329,共4页Chinese Pharmacological Bulletin

基　　金：广东省卫生厅科研基金项目 NoA199945 2

摘　　要：目的　观察特乐定与固邦联合用药是否对大鼠骨骼产生增强疗效的作用。方法　 3mon龄♀SD大鼠 4 2只 ,体重 (2 6 6± 2 2 ) g ,随机分为 6组 ,除了第 1组实行Sham手术外 ,其余 5组均实行去卵巢 (Ovx)手术。Sham大鼠给予蒸馏水 ,各Ovx大鼠分别给予蒸馏水、1mg·kg-1·d-1固邦、2 70mg·kg-1·d-1的葡萄糖酸钙、5 6mg·kg-1·d-1的特乐定及结合 1mg·kg-1·d-1固邦与 5 6mg·kg-1·d-1的特乐定。实验期限为 3mon ,处死前注射荧光标记。实验结束后取左侧胫骨近心端 ,行骨组织形态计量学分析。结果　固邦增加大鼠骨量 ,抑制去卵巢大鼠的骨形成指标 (%L .Pm ,BFR/BS)和骨吸收指标 (N .Oc/Tb .Pm)。单独补充葡萄糖酸钙未能缓解Ovx大鼠的骨量丢失。特乐定亦未能有效恢复Ovx大鼠丢失的骨量。固邦与特乐定联合用药虽然增加去卵巢大鼠的骨量 ,但其骨量不比单用固邦的骨量高。结论　固邦与特乐定的联合应用 ,不能发生疗效增强的效果 。AIM To evaluate whether l glutamine monofluorophosphate(MFP) together with Alendronate sodium may further increase bone mass in ovariectomized rats. METHODS Forty two 3 month old Sprague Dawley female rats were randomized into six groups:group 1 rats were sham operated(Sham),group 2 rats were ovariectomized controls(Ovx),and groups 3～6 were ovariectomized and received either 1 mg·kg -1 ·d -1 of alendronate sodium ,270 mg·kg -1 ·d -1 of calcium gluconate,5 6 mg·kg -1 ·d -1 of MFP or combination of 5 6 mg·kg -1 ·d -1 of MFP and 1 mg·kg -1 ·d -1 of alendronate sodium for 3 months. All animals received double bone fluorochrome labeling prior to sacrifice. At the end of experiment,the left tibiae were havested for histomorphometrical evaluations. RESULTS Alendronate sodium increased trabecular bone volume significantly with suppressed bone resorption and bone formation. Administrated calcium gluconate had no influence on the bone mass. MFP also could not restore cancellous bone of ovariectomized rats. Administration of both MFP and alendronate sodium increased bone mass significantly but did not increase bone mass compared with alendronate sodium. CONCLUSIONS The results indicated administration of both alendronate sodium and MFP could not further increase bone mass of ovariectomized rats.

关 键 词：特乐定 固邦 去卵巢大鼠 骨质疏松 骨组织形态计量学 

分 类 号：R711.75[医药卫生—妇产科学] R681[医药卫生—临床医学]