木瓜苷对大鼠佐剂性关节炎的治疗作用  

Therapeutic effect of glucosides of chaenomeles speciosa on adjuvant arthritis in rats

作　　者：戴敏[1] 魏伟[2] 汪倪萍[2] 陈群[2] 

机构地区：[1]安徽中医学院药学系中药药理学教研室,合肥230038 [2]安徽医科大学临床药理研究所,合肥230032

出　　处：《中国药理学通报》2003年第3期340-344,共5页Chinese Pharmacological Bulletin

基　　金：安徽省自然科学基金资助课题No 0 0 0 44 411;安徽省"十五"科技重大专项资金资助课题No 0 180 3 0 2 6;合肥市重点科技项目资助课题No 2 0 0 1 48 14

摘　　要：目的　研究木瓜苷对佐剂性关节炎 (AA)大鼠的治疗作用及部分机制。方法　大鼠足跖皮内注射弗氏完全佐剂诱发大鼠AA模型 ;足容积法测量继发侧足肿胀度 ,进行疼痛评分和多发性关节炎评分 ,MTT法检测胸腺T淋巴细胞和脾脏B淋巴细胞的增殖反应以及腹腔巨噬细胞产生IL 1水平 ,放射性免疫法测定腹腔巨噬细胞产生TNFα 和PGE2含量。结果　大鼠免疫后d 17开始分别灌胃木瓜苷 30、6 0、12 0mg·kg-1和阿克他利 6 0mg·kg-1对照药 ,连续给药 8d。木瓜苷 6 0、12 0mg·kg-1于d 2 1、d 2 4降低AA大鼠继发侧关节肿胀度、关节疼痛评分、多发性关节炎指数 ;木瓜苷 12 0mg·kg-1恢复AA大鼠胸腺T淋巴细胞增殖能力 ;木瓜苷各剂量还可不同程度抑制AA大鼠腹腔巨噬细胞产生IL 1、TNFα 和PGE2 。结论　木瓜苷可减轻AA大鼠关节肿胀、疼痛和多发性关节炎程度 ;该作用可能与调节T淋巴细胞的功能 。AIM To investigate the therapeutic effect and mechanism of glucosides of Chaenomeles speciosa (GCS) on adjuvant arthritis (AA) in rats. METHODS Complete Freund's adjuvant was used to induce AA in rats. Secondary paw swelling of AA rats was measured with volume meter. Pain response and polyarthritis index were scored. Meanwhile, ConA induced thymocyte proliferation and LPS induced splenocyte proliferation were assayed by 3 (4,5 dimethylthiazol 2 thiazolyl) 2,5 diphenyl 2H tetrazolium bromide (MTT) assay. IL 1 production was measured by thymocyte proliferation assay; TNF α and PGE 2 production were determined by radio immunoassay. RESULTS GCS (30, 60, 120 mg·kg -1 ) and Actarit (60 mg·kg -1 ) were given by intragastric administration for 8 days from the 17th day after immunization. Treatment with GCS (60, 120 mg·kg -1 ) and Actarit (60 mg·kg -1 ) for 5 days significantly diminished the secondary hind paw swelling, as well as relieved the pain response and the polyarthritic symptoms of the whole body as compared with AA model group. GCS (120 mg·kg -1 ) and Actarit (60 mg·kg -1 ) restored the diminished thymocytes proliferation ConA induced in AA rats. GCS reduced the enhanced productions of IL 1,TNF α and PGE 2 from peritoneal macrophages in AA rats. CONCLUSION GCS has therapeutic effect on AA in rats, which may be related to regulation function of thymocyte T cells and inhibiting the productions of proimflammatory cytokines secreted by peritoneal macrophages.

关 键 词：木瓜苷 佐剂性关节炎 T淋巴细胞 腹腔巨噬细胞 大鼠 

分 类 号：R274.943[医药卫生—中西医结合]