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机构地区:[1]广西植物研究所,桂林541006
出 处:《广西植物》1992年第1期59-63,共5页Guihaia
摘 要:本文报道以广西四个县所产绞股蓝,在MS基本培养基中进行茎段培养,研究植物激素对器官形成的影响。试验结果表明,细胞分裂素BA明显促进芽的形成和增殖,BA和NAA或GA配合使用有助于苗的形成和生长,不同产地的绞股蓝类型均能诱导形成丛生芽,但分化芽数有差异。诱导生根用1/2 MS附加NAA或IBA,试管苗一年四季移栽均获得较高的成活率。幼苗移栽大田生长良好,当年可获得较好产量。This paper reports the rapid propagation of Gynostemma pentaphyllum (Thunb.) Makino from four counties of Guangxi; their stem explants were grown on MS basal medium and the effects of plant hormones on organogenesis were studied. The results showed that BA markedly stimulated the bud formation and proliferation. The use of combination of BA and NAA or GA is beneficial to shoots formation and growth. All types of Gynostemma pentaphyllum (Thunb.) Makino from different places induced many buds formation, but the number of bud formation were different. The use of 1/2MS basal medium with NAA or IBA is beneficial to induce root formation. The survival rate of test-tube plantlets transplanting always obtain quite high at all seasons. The plants were grown well in field and great yield were gained in the same year.
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