茄子抗冷细胞变异体的RAPD分析及自交一代株系的抗冷性鉴定  

RAPD Analysis and Identification of Chilling Resistance of Cellular Variation in Eggplant

作　　者：赵福宽[1] 高遐虹[1] 程继鸿[1] 范双喜[1] 于颖[1] 

机构地区：[1]北京农学院园艺系,北京102206

出　　处：《华北农学报》2003年第1期17-19,共3页Acta Agriculturae Boreali-Sinica

基　　金：北京市自然科学基金资助项目(5982006)

摘　　要：以茄子品系E 9903花药低温胁迫培养获得的抗冷细胞变异体为试材,用SDS方法从茄子幼嫩叶片提取DNA,选用40个长度为10个碱基的寡聚核苷酸(OPK01-OPK20,OPJ01-OPJ20)作为RAPD引物进行PCR扩增反应,并测定抗冷细胞变异体自交一代株系的冷害指数。结果表明:以OPK12为PCR反应引物时RAPD扩增谱带在抗冷性植株与同品系的对照株之间存在多态性差异,抗冷细胞变异体自交一代株系的冷害指数显著低于同品系的对照。Chillingresistant plants regenerated from anther of eggplant strain E9903 under low temperature stress culture were used as experimental material.DNA was extracted from young leaves using SDS method.Forty 10baselong oligonucleotide primers were used in polymerase chain reaction.The index of chilling injury of cellular variation S1 progeny were measured.Results showed that polymorphism existed when primer OPK12 was used in polymerase chain reaction.The index of chilling injury of cellular variation S1 progeny were much lower than that of control strain.

关 键 词：茄子 抗冷细胞 变异体 RAPD分析 自交一代株系 抗冷性鉴定 

分 类 号：S641.1[农业科学—蔬菜学]