链球菌组蛋白样蛋白对小鼠腹腔巨噬细胞产生IL-6的影响  

Streptococcal Histone-Like Protein Induces Murine Peritoneal MΦto Produce IL-6

作　　者：沈海中[1] 张力平[1] 平国玲[1] 张红春[1] 靖学芳[1] 李郁英[1] 李玉兰[1] 李卫红[2] 张海燕[2] 

机构地区：[1]首都医科大学免疫学系 [2]首都医科大学细胞生物系

出　　处：《首都医科大学学报》2003年第1期11-13,共3页Journal of Capital Medical University

基　　金：北京市自然科学基金资助项目 ( 7992 0 0 3 )

摘　　要：为研究链球菌组蛋白样蛋白 (HlpA)对小鼠腹腔巨噬细胞 (MΦ)体外产生白细胞介素 6(IL 6)的影响 ,将不同质量浓度的HlpA和 (或 )LTA放入培养的细胞中 ,作用不同时间后收集上清液 ,用IL 6依赖株 7TD1细胞测定样品IL 6活性。结果显示 ,链球菌HlpA( 0～ 1 0 0mg/L)在体外以剂量和时间依赖方式促进MΦ产生IL 6。LTA和HlpA的混合物对IL 6的产生呈协同增强作用 ;To evaluate the effect of Streptococcus HlpA on interlukin 6(IL 6)production by murine peritoneal MΦ, HlpA and/or LTA were added to the cultured MΦ and maintained for different time periods. IL 6 activity was measured by means of 7TD1 cells. The results showed that the IL 6 production from MΦ induced by HlpA of Streptococcus was time and concentration dependent in vitro. There was a synergisticly increased response on the production of IL 6 induced by the mixture of HlpA and LTA, as compared to HlpA alone. On the contrary, the addition of heparin could deduce the IL 6 production.

关 键 词：链球菌 巨噬细胞 白细胞介素6 

分 类 号：R378.12[医药卫生—病原生物学]