救脑益智胶囊水提液对半乳糖老化小鼠海马神经元损伤的保护作用  

Protection of Liquid Extract of Jiunaoyizhi Capsule Against Impairment of Hippocampal Neurons of D-galactose-Induced Aging Mice

作　　者：赵志炜[1] 王蓬文[2] 宋丽娜[2] 庄晓明[1] 王元身[3] 姬志娟[1] 盛树力[1] 

机构地区：[1]首都医科大学宣武医院北京脑老化重点实验室 [2]首都医科大学病理系 [3]首都医科大学北京神经科学研究所

出　　处：《首都医科大学学报》2003年第1期33-36,共4页Journal of Capital Medical University

基　　金：国家科技部 973课题资助项目 (G2 0 0 0 0 5 70 0 5 )

摘　　要：为观察救脑益智水提液对半乳糖老化小鼠海马神经元的神经元存活信号转导通路和凋亡级联反应中某些蛋白质表达的影响 ,将昆明种小鼠分为 4组 (每组 2 4只 ) :正常对照组 (C组 )、D 半乳糖老化模型组 (D组 )、大剂量药物治疗组 (B组 )和小剂量药物治疗组 (S组 )。除C组外 ,3组小鼠每日皮下注射半乳糖 5 0mg/kg。其中B组和S组每日分别灌胃救脑益智胶囊水提取液 ,相当于生药 1 g/kg和 0 .3 g/kg ,C组和D组分别灌胃等量生理盐水 ,共 3个月。结果 :D组小鼠海马神经元中Akt/PKB(蛋白激酶B)、CREB(cAMPresponseelementbindingprotein)和Bcl 2的免疫组化阳性细胞数明显减少 ,而AIF(凋亡诱导因子 )和Bax明显增加 ,PP 1 (蛋白磷酸酯酶 1 )也明显减少。S组上述改变恢复正常 ,而B组却无此作用。提示 :小剂量救脑益智水提液对半乳糖老化模型小鼠海马神经元的存活信号转导通路和凋亡级联反应的改变具有恢复正常的作用 ,而大剂量则无此作用。The objective of this study was to investigate whether the liquid extract of Jiunaoyizhi capsule has a protective effect on neuronal impairment of the aging mice induced by D galactose. Several antibodies were applied to detect the immunoreactive positive neurons in hippocampus. It was found that in the D galactose induced aging mice the Akt/PKB, CREB and Bcl 2 is as well as PP 1 positive neurons decreased obviously, but AIF and Bax increased significantly. The mice treated with 0.3 g/kg liquid extract of raw herbs could make these changes normal. The small dosage of jiunaoyizhi has effect on the impairment of signal pathway of hippocampal neurons in the aging mice. But the unexpected result was that the 1.0 g/kg liquid extract exerted no such effect.

关 键 词：救脑益智胶囊 半乳糖老化小鼠 海马神经元 信号转导 

分 类 号：R285.6[医药卫生—中药学]