多核细胞法和Brdurd法检测辐射诱发体细胞HPRT基因突变的比较研究  

Comparative Study on Multinucleated Cell Assay and Brdurd Labeling in Detecting the Somatic HPRT Gene Mutation Induced by Radiation

作　　者：赵经涌[1] 崔凤梅[1] 劳勤华[1] 徐永忠[1] 赵涛[1] 

机构地区：[1]苏州大学放射医学系,江苏苏州215007

出　　处：《工业卫生与职业病》2003年第2期72-74,共3页Industrial Health and Occupational Diseases

摘　　要：目的　研究放射性核素内照射诱发体细胞HPRT基因位点突变及其剂量 -效应关系 ,比较不同方法对HPRT基因位点突变的检出率。方法　给大鼠尾静脉注射晚期混合裂变产物后不同时间心脏穿刺取血。用多核细胞法和Brdurd法分别检测HPRT基因突变细胞 ,计算突变率 ,拟合出辐射诱发HPRT基因位点突变的剂量 -效应关系函数。结果　用多核细胞法检测的放射性核素内照射诱发HPRT基因位点突变率 (y ,‰ )和剂量 (D ,cSv)相关函数为y =1 14 98+0 1199lnD,r =0 970 6 ;用Brdurd法检测的相关函数为 y =6 5 310× 10 -2 +3 4 5 4 2× 10 -3 D ,r =0 984 6。多核细胞法检测HPRT基因位点突变检出率比Brdurd法高 11～ 17倍之多。结论　体细胞HPRT基因对电离辐射敏感 ,有可能作为辐射生物剂量计。Objective To study the HPRT gene locus mutation in peripheral blood lymphocytes induced by internal exposure to radionuclides and the relationship between mutation frequency and dosage.Mutation detecting rate by different methods was also compared.Methods Rats were injected intravenously with radionuclides.Blood was sampled at different time after injection.HPRT gene mutation frequency was examined by multinucleated cell assay and Brdurd labeling respectively and its dose response function was derived.Results For multinucleated cell assay and Brdurd labeling,the dose response functions for HPRT gene locus mutation with mutation frequency(y,‰)and dose(D,cSv)were y=1 149 8+0 119 9ln D,r=0 970 6 and y=6 531 0×10 -2 +3 454 2× 10 -3 D, r=0 984 6 respectively.Mutation detecting rate by multinucleated cell assay is 11～17 times than that by Brdurd labeling method.Conclusions HPRT gene locus mutation is very sensitive to radiation and may be served as a biological dosimeter.Mutation detecting rate by multinucleated cell assay is higher than by Brdurd labeling method.

关 键 词：放射性核素 内照射 HPRT基因突变 多核细胞法 Brdurd法 

分 类 号：R114[医药卫生—卫生毒理学]