中国人丙型肝炎病毒全长NS3基因的克隆、序列分析及表达  

Cloning and Expression of Full-length of Chinese HCV NS3 Gene in E.coli

作　　者：王尊文[1] 祁自柏[1] 谷金莲[1] 于洋[1] 杨振[1] 张华远[1] 李河民[1] 

机构地区：[1]中国药品生物制品检定所,北京100050

出　　处：《中国生物制品学杂志》2003年第2期65-67,共3页Chinese Journal of Biologicals

摘　　要：目的　为改进HCV试剂的质量克隆 1b型丙型肝炎病毒 (HCV)全长NS3基因并在原核细胞中表达。方法　用RT PCR方法从中国人血清中扩增全长NS3片段 ,进行序列分析 ,并克隆到pET3 0a(+)原核表达载体中 ,构建的原核表达载体pET NS3 180 0在大肠杆菌BL2 1(DE3 )中表达 ,用Westernblot方法进行验证。结果　扩增到 1b型HCV全长NS3片段 ,经Westernblot实验表明该片段具有抗原活性。Objective To clone the full length of Chinese HCV NS3 gene and express it in E.coli. Methods Amplify the full length of NS3 gene fragment from Chinese serum,sequence,clone into expression vector pET30a(+),and express in E.coli BL21(DE3).The expressed product was identified by Western blot.Results The full length of HCV NS3 gene fragment was amplified.Western blot showed antigenicity of the expressed product.Conclusion The constructed plasmid can be used for the expression of NS3 protein. ;

关 键 词：中国人 丙型肝炎病毒 NS3基因 克隆 序列分析 表达 

分 类 号：R373.21[医药卫生—病原生物学]