去氧胆酸钠裂解流感病毒的效果研究  

Lysis of Influenza Virus by Sodium Deoxycholate

作　　者：戚凤春[1] 王敏文[1] 薄洪[1] 郭桥[1] 范宇红[1] 王志刚[1] 张玉辉[1] 阎长太[1] 盛军[1] 

机构地区：[1]长春生物制品研究所,长春130062

出　　处：《中国生物制品学杂志》2003年第2期97-99,共3页Chinese Journal of Biologicals

摘　　要：目的　研究去氧胆酸钠对流感病毒最佳的裂解条件。方法　流感病毒在不同浓度的去氧胆酸钠作用一定时间后 ,经蔗糖梯度密度离心纯化 ,制备出流感裂解疫苗样品。以电镜观察病毒裂解情况和血液凝集反应 ,测定病毒效价作为评价指标 ,确定最佳裂解条件。结果　裂解纯化后的样品经电镜、SDS PAGE检测表明病毒颗粒完全裂解 ,裂解后的血凝效价收率约为 80 %。结论　 0 .8%的去氧胆酸钠作用 12 0min可使流感病毒达到最佳裂解效果。Objective To determine the optimal condition of lysis of influenza virus by sodium deoxycholate.Methods Lyse infulenza virus by the sodium deoxycholate at different concentrations and purify by sucrose density gradient centrifugation.Observe the lysis of virus by electron microscopy and detect virus titers by hemagglutination reaction.Results Electron microscopy and SDS PAGE proved that the virus particles were completely lysed,and about 80% of its titer was remained.Conclusion The optimal concentration of sodium deoxycholate is 0.8%,and the optimal reaction time is 120 minutes.

关 键 词：去氧胆酸钠 裂解 血凝素 流感病毒 

分 类 号：R373.13[医药卫生—病原生物学]