8种唇口目苔藓动物的分子系统发生——基于28S rRNA基因的证据  

THE MOLECULAR PHYLOGENY OF EIGHT SPECIES OF CHEILOSTOME BRYOZOANS BASED ON PARTIAL 28S rRNA GENE SEQUENCES

作　　者：郝家胜[1] 

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000

出　　处：《安徽师范大学学报（自然科学版）》2003年第1期51-54,共4页Journal of Anhui Normal University(Natural Science)

基　　金：中国科学院南京地质古生物所现代古生物学和地层学国家重点实验室开放基金 (0 13 115 );博士后流动站基金资助课题

摘　　要：对我国沿海较为常见的 8种唇口目苔藓动物的 2 8SrRNA基因的部分序列进行了PCR扩增和序列测定 .用排序软件进行序列比对后 ,以腕足动物为外组群 ,运用MEGA遗传分析软件以邻接法 (NJ)和最大似然法 (MP)构建了系统发生树 .结果表明 ,有囊亚目的 4种苔藓动物 (刺轴拟缘孔苔虫、假缘孔苔虫、拟迷误裂孔苔虫、仿分胞苔虫 )构成一个单系群 ;无囊亚目则是一个复系群 ,其中 2种草苔虫 (匍茎草苔虫、多室草苔虫 )为单系发生 。s: The partial sequences of 28S rRNA gene from eight species of cheilostome bryozoans commonly found in the coastal waters of China were amplified through the polymerase chain reaction (PCR) and the PCR products were sequenced directly with ABI 377 DNA automati sequencer. Terebratella tranversa as an outgroup, we constructed their phylogenetic trees with neigbour joining (NJ) and maximum parsimony (MP) methods using MEGA (Version 2.0) software. The results show that the four species of Ascophora (Smittoidea spinigera, Parasmittina sp., Schizoaporella erratoidea, Celleporina sp.) constitute a monophyletic group; whereas the four species of Anasca make up a polyphyletic group, except for Bugula neritina and Bugula stolonifera is monophyletic. As for Membranipora grandicella and Electra bellula, further studies on other molecular phylogenies are needed to ascertain their phylogentic positions.

关 键 词：分子系统发生 28S RRNA基因 唇口目 苔藓动物 PCR扩增 序列测定 腕足动物 

分 类 号：Q959.23[生物学—动物学]