固相萃取高效液相法测定人血浆中格列吡嗪浓度  

Determination of glipizide in plasma by HPLC using solid phase extraction

作　　者：钟明康[1] 刘洋波[2] 施孝金[1] 张莉莉[1] 张静华[1] 

机构地区：[1]复旦大学附属华山医院临床药学研究室,上海200040 [2]福建省泉州市第一医院药剂科,泉州362000

出　　处：《中国临床药学杂志》2003年第2期70-72,共3页Chinese Journal of Clinical Pharmacy

摘　　要：目的 :建立人血浆中格列吡嗪 (Gli)浓度的测定方法。方法 :采用固相萃取法处理血样 ,HPLC法测定 ,流动相 0 0 4mol·L-1KH2 PO4 乙腈 异丙醇 (60∶3 0∶10 ,V/V) ,pH 3 2 0 ,流速 1 2 5mL·min-1,柱温 3 0℃ ,分析柱Kromasil10 0 5C18(15 0mm× 4 6mm ,5 μm) ,检测波长 2 2 7nm。结果 :线性范围 2 0～ 10 0 0ng·mL-1(r =0 9999) ,平均提取回收率 >83 5 % ,批内、批间RSD <5 0 %。结论 :本法测定人血浆中Gli浓度稳定、灵敏、可靠 。AIM: To develop HPLC for the determination of glipizide in human plasma. METHODS: The sample was determined by HPLC using solid phase extraction. Using a chemical bonded column(Kromasil 100 5 C 18 , 150 mm×4 6 mm, 5 μm) with temperature of 30 ℃. The detected wavelength was set at 227 nm. The mobile phase was 0 04 mol·L -1 monopotassium phosphate acetonitrile isopropyl alcohol (60:30:10,V/V,pH=3 20) with a flow rate 1 25 mL· min -1 . RESULTS: Linear responses in analyte/internal standard peak area ratios were observed for analyte concentrations ranging from 2 ng·mL -1 to 1 000 ng·mL -1 ( r =0 999 9). The mean recovery was 83 5%, The relative standard deviation of within day and between day were limited 5 0%, respectively. CONCLUSION: This method to determination of glipizide in plasma is stable, sensitive and accurate, and suitable for pharmacokinetic study clinically.

关 键 词：固相萃取 高效液相法 测定 人血浆 格列吡嗪 降糖药 

分 类 号：R96[医药卫生—药理学]